LOCUS       sequence-147858-       13457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression, lentiviral packaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pCMV-deltaR8.2(A14C,E45C,A92E)
FEATURES             Location/Qualifiers
     source          1..13457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        26..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..609
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     560..580
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     606..630
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             880..2382
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQCISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSCGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IEPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2175..5186
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFTQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDGLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4871..4988
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6557..6790
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6975..7019
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7313..7339
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        complement(8576..8594)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8577..8594)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8885..8904)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9119..9138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9238..9260
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        9384..9488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9489..9557,9558..10349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9707..9726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10520..11108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11009..11028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11262..11279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11349..11369)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11354..11683
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11534..11669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12818..12883
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13013..13033
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gggctgcagg aattcgagct cgcccgacat tgattattga ctagttatta atagtaatca
       61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      601 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      661 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
      721 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
      781 ttggcttctt atgcgacgga tcgatcccgt aataagcttc gaggtccgcg gccgcgttga
      841 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta
      901 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa
      961 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     1021 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     1081 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     1141 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1201 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag
     1261 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcagtgcata
     1321 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa
     1381 gtgataccca tgttttcagc attatcatgc ggagccaccc cacaagattt aaacaccatg
     1441 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag
     1501 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattga accaggccag
     1561 atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca ggaacaaata
     1621 ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag atggataatc
     1681 ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga cataagacaa
     1741 ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct aagagccgag
     1801 caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca aaatgcgaac
     1861 ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga agaaatgatg
     1921 acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc tgaagcaatg
     1981 agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag gaaccaaaga
     2041 aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa ttgcagggcc
     2101 cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa agattgtact
     2161 gagagacagg ctaatttttt agggaagatc tggccttcac acaagggaag gccagggaat
     2221 tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag gtttggggaa
     2281 gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta tcctttagct
     2341 tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg gggcaattaa
     2401 aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg aatttgccag
     2461 gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta agacagtatg
     2521 atcagatact catagaaatc tgcggacata aagctatagg tacagtatta gtaggaccta
     2581 cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact ttaaattttc
     2641 ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat ggcccaaaag
     2701 ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt tgtacagaaa
     2761 tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat actccagtat
     2821 ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc agagaactta
     2881 ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct gcagggttaa
     2941 aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca gttcccttag
     3001 ataaagactt caggaagtat actgcattta ccatacctag tataaacaat gagacaccag
     3061 ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca gcaatattcc
     3121 agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac atagtcatct
     3181 atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag catagaacaa
     3241 aaatagagga actgagacaa catctgttga ggtggggatt taccacacca gacaaaaaac
     3301 atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat aaatggacag
     3361 tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata cagaaattag
     3421 tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg caattatgta
     3481 aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa gaagcagagc
     3541 tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg tattatgacc
     3601 catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg acatatcaaa
     3661 tttatcaaga gccatttaaa aatctgaaaa caggaaaata tgcaagaatg aagggtgccc
     3721 acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca gaaagcatag
     3781 taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca tgggaagcat
     3841 ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc aatacccctc
     3901 ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca gaaactttct
     3961 atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat gtaactgaca
     4021 gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact gagttacaag
     4081 caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca gactcacaat
     4141 atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta gtcagtcaaa
     4201 taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca gcacacaaag
     4261 gaattggagg aaatgaacaa gtagatgggt tggtcagtgc tggaatcagg aaagtactat
     4321 ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt aattggagag
     4381 caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta gccagctgtg
     4441 ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc ccaggaatat
     4501 ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt catgtagcca
     4561 gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca gcatacttcc
     4621 tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat ggcagcaatt
     4681 tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag gaatttggca
     4741 ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa ttaaagaaaa
     4801 ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat
     4861 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     4921 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     4981 aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga ccagcaaagc
     5041 tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc
     5101 caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt gatgattgtg
     5161 tggcaagtag acaggatgag gattaacaca tggaaaagat tagtaaaaca ccatatgtat
     5221 atttcaagga aagctaagga ctggttttat agacatcact atgaaagtac taatccaaaa
     5281 ataagttcag aagtacacat cccactaggg gatgctaaat tagtaataac aacatattgg
     5341 ggtctgcata caggagaaag agactggcat ttgggtcagg gagtctccat agaatggagg
     5401 aaaaagagat atagcacaca agtagaccct gacctagcag accaactaat tcatctgcac
     5461 tattttgatt gtttttcaga atctgctata agaaatacca tattaggacg tatagttagt
     5521 cctaggtgtg aatatcaagc aggacataac aaggtaggat ctctacagta cttggcacta
     5581 gcagcattaa taaaaccaaa acagataaag ccacctttgc ctagtgttag gaaactgaca
     5641 gaggacagat ggaacaagcc ccagaagacc aagggccaca gagggagcca tacaatgaat
     5701 ggacactaga gcttttagag gaacttaaga gtgaagctgt tagacatttt cctaggatat
     5761 ggctccataa cttaggacaa catatctatg aaacttacgg ggatacttgg gcaggagtgg
     5821 aagccataat aagaattctg caacaactgc tgtttatcca tttcagaatt gggtgtcgac
     5881 atagcagaat aggcgttact cgacagagga gagcaagaaa tggagccagt agatcctaga
     5941 ctagagccct ggaagcatcc aggaagtcag cctaaaactg cttgtaccaa ttgctattgt
     6001 aaaaagtgtt gctttcattg ccaagtttgt ttcatgacaa aagccttagg catctcctat
     6061 ggcaggaaga agcggagaca gcgacgaaga gctcatcaga acagtcagac tcatcaagct
     6121 tctctatcaa agcagtaagt agtacatgta atgcaaccta taatagtagc aatagtagca
     6181 ttagtagtag caataataat agcaatagtt gtgtggtcca tagtaatcat agaatatagg
     6241 aaaatattaa gacaaagaaa aatagacagg ttaattgata gactaataga aagagcagaa
     6301 gacagtggca atgagagtga aggagaagta tcagcacttg tggagatggg ggtggaaatg
     6361 gggcaccatg ctccttggga tattgatgat ctgtagttga gcggccgctg atcttcagac
     6421 ttggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6481 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6541 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6601 tgggcgcagc ctcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     6661 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     6721 tctggggcat caagcagctc caagcaagaa tcctagctgt ggaaagatac ctaaaggatc
     6781 aacagctcct agggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     6841 ggaatgctag ttggagtaat aaatctctgg aacagatctg gaatcacacg acctggatgg
     6901 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     6961 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7021 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7081 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     7141 agggatattc accattatcg tttcagaccc acctcccaat cccgagggga cccgacaggc
     7201 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     7261 acggatcctt ggcacttatc tgggacgatc tgcggagcct gtgcctcttc agctaccacc
     7321 gcttgagaga cttactcttg attgtaacga ggattgtgga acttctggga cgcagggggt
     7381 gggaagccct caaatattgg tggaatctcc tacaatattg gagtcaggag ctaaagaata
     7441 gtgctgttag cttgctcaat gccacagcca tagcagtagc tgaggggaca gatagggtta
     7501 tagaagtagt acaaggagct tgtagagcta ttcgccacat acctagaaga ataagacagg
     7561 gcttggaaag gattttgcta taagatgggt ggcaagtggt caaaaagtag tgtgattgga
     7621 tggcctactg taagggaaag aatgagacga gctgagccag cagcagatgg ggtgggagca
     7681 gtatctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7741 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7801 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7861 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7921 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     7981 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     8041 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     8101 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     8161 cacatggccc gagagctgca tccggagtac ttcaagaact gctgagcggc cgccccggtg
     8221 accttcagac cttggcactg gaggtggccc ggcagaagcg cggcatcgtg gatcagtgct
     8281 gcaccagcat ctgctctctc taccaactgg agaactactg caactaggcc caccactacc
     8341 ctgtccaccc ctctgcaatg aataaaacct ttgaaagagc actacaagtt gtgtgtacat
     8401 gcgtgcatgt gcatatgtgg tgcgggggga acatgagtgg ggctggctgg agtggcgatg
     8461 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
     8521 ataggttaat gtcatgataa taatggtttc ttagtctaga attaattccg tgtattctat
     8581 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     8641 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     8701 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     8761 gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     8821 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     8881 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     8941 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     9001 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     9061 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gatatggtgc actctcagta
     9121 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     9181 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9241 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     9301 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9361 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    10681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11281 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11341 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    11401 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    11461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    11521 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    11581 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    11641 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
    11701 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
    11761 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
    11821 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
    11881 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
    11941 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
    12001 tggtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    12061 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    12121 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
    12181 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    12241 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
    12301 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
    12361 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
    12421 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    12481 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    12541 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    12601 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    12661 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
    12721 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
    12781 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
    12841 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
    12901 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
    12961 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
    13021 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
    13081 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
    13141 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
    13201 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
    13261 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
    13321 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
    13381 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    13441 atgcaattgt tgttgtt
//
