LOCUS       sequence-147880-        1285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-RC3AI
FEATURES             Location/Qualifiers
     source          1..1285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(505..523)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     773..792
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgaat tgccaccatg tacccctacg acgtgcccga ctacgccatc aagatcgcca
       61 ccaggaagta cctgggcaag cagaacgtgt acgacatcgg cgtggagagg gaccacaact
      121 tcgccctgaa gaacggcttc atcgccagca actgcttcaa cgaattcatg ggcgccctga
      181 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      241 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      301 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      361 agggccgcca ctccaccgac gaggtggacg gcatcatcaa ggagttcatg cgcttcaagg
      421 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      481 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      541 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      601 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      661 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      721 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      781 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggat
      841 ccgccgagta ctgcctgagc tacgagaccg agatcctgac cgtggagtac ggcctgctgc
      901 ctatcggcaa gatcgtggag aagaggatcg agtgcaccgt gtacagcgtg gacaacaacg
      961 gcaacatcta cacccagcct gtggcccagt ggcacgacag gggcgagcag gaggtgttcg
     1021 agtactgcct ggaggacggc agcctgatca gggccaccaa ggaccacaag ttcatgaccg
     1081 tggacggcca gatgctgcct atcgacgaga tcttcgagag ggagctggac ctgatgaggg
     1141 tggacaacct gcctaacccc gacttcccac ccgaagtaga ggaacaggat gccagcaccc
     1201 tgcctgtgtc ttgtgcctgg gagagtggga tgaaacgcca cagagcagcc tgtgcttcgg
     1261 ctagtattaa tgtgtaagcg gccgc
//
