LOCUS       sequence-147881-        1219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-CC3AI
FEATURES             Location/Qualifiers
     source          1..1219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     359..380
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(438..459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(699..718)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1179..1208
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctagcgaat tgccaccatg tacccctacg acgtgcccga ctacgccatc aagatcgcca
       61 ccaggaagta cctgggcaag cagaacgtgt acgacatcgg cgtggagagg gaccacaact
      121 tcgccctgaa gaacggcttc atcgccagca actgcttcaa cgaattcatg gacaagcaga
      181 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
      241 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      301 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
      361 tggtcctgct ggagttcgtg accgccgccg ggatcgacga ggtggacggc gagctgttca
      421 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      481 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      541 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
      601 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      661 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      721 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      781 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc atcagcgaca
      841 acgtctatat caccgccgga tccgccgagt actgcctgag ctacgagacc gagatcctga
      901 ccgtggagta cggcctgctg cctatcggca agatcgtgga gaagaggatc gagtgcaccg
      961 tgtacagcgt ggacaacaac ggcaacatct acacccagcc tgtggcccag tggcacgaca
     1021 ggggcgagca ggaggtgttc gagtactgcc tggaggacgg cagcctgatc agggccacca
     1081 aggaccacaa gttcatgacc gtggacggcc agatgctgcc tatcgacgag atcttcgaga
     1141 gggagctgga cctgatgagg gtggacaacc tgcctaacga gcagaagctg atcagcgagg
     1201 aggacctgta agcggccgc
//
