LOCUS       sequence-147882-        1213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-VC3AI
FEATURES             Location/Qualifiers
     source          1..1213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     359..380
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(432..453)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(693..712)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1173..1202
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctagcgaat tgccaccatg tacccctacg acgtgcccga ctacgccatc aagatcgcca
       61 ccaggaagta cctgggcaag cagaacgtgt acgacatcgg cgtggagagg gaccacaact
      121 tcgccctgaa gaacggcttc atcgccagca actgcttcaa cgaattcatg gacaagcaga
      181 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
      241 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      301 accactacct gagctaccag tccaaactga gcaaagaccc caacgagaag cgcgatcaca
      361 tggtcctgct ggagttcgtg accgccgccg ggatcgacga ggtggacggc ttcaccgggg
      421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
      541 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
      601 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      841 atatcaccgc cggatccgcc gagtactgcc tgagctacga gaccgagatc ctgaccgtgg
      901 agtacggcct gctgcctatc ggcaagatcg tggagaagag gatcgagtgc accgtgtaca
      961 gcgtggacaa caacggcaac atctacaccc agcctgtggc ccagtggcac gacaggggcg
     1021 agcaggaggt gttcgagtac tgcctggagg acggcagcct gatcagggcc accaaggacc
     1081 acaagttcat gaccgtggac ggccagatgc tgcctatcga cgagatcttc gagagggagc
     1141 tggacctgat gagggtggac aacctgccta acgagcagaa gctgatcagc gaggaggacc
     1201 tgtaagcggc cgc
//
