LOCUS       sequence-147886-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG 
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Saarimaki-Vire J, Balboa D, Russell MA, Saarikettu J, Kinnunen M, 
            Keskitalo S, Malhi A, Valensisi C, Andrus C, Eurola S, Grym H, 
            Ustinov J, Wartiovaara K, Hawkins RD, Silvennoinen O, Varjosalo M, 
            Morgan NG, Otonkoski T
  TITLE     An Activating STAT3 Mutation Causes Neonatal Diabetes through 
            Premature Induction of Pancreatic Differentiation.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):281-294. doi: 
            10.1016/j.celrep.2017.03.055.
  PUBMED    28402852
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "281-294"
COMMENT     Sequence Label: CAG-Cas9-T2A-EGFP-ires-puro
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             71..136
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             143..163
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaccgg
       61 tgccaccatg gactataagg accacgacgg agactacaag gatcatgata ttgattacaa
      121 agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc
      181 agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
      241 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
      301 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
      361 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
      421 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
      481 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
      541 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
      601 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
      661 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
      721 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
      781 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
      841 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
      901 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
      961 cgaggatgcc aaa
//
