LOCUS       sequence-147887-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the double Holliday junction 
            substrate (DHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Plank JL, Hsieh TS
  TITLE     A novel, topologically constrained DNA molecule containing a double 
            Holliday junction: design, synthesis, and initial biochemical 
            characterization.
  JOURNAL   J Biol Chem. 2006 Jun 23;281(25):17510-6. Epub 2006 Apr 11.
  PUBMED    16608853
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-06-23"; volume: "281(25)"; pages: "17510-6. Epub
            2006 Apr 11"
COMMENT     Sequence Label: pDHJS3 AS+
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     188..210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     202..219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     203..219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..245
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        226..244
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     269..285
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     270..286
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(300..333)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(765..798)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(822..840)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(861..877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(861..877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(874..896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    885..901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(909..915,916..933,934..939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    954..975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
       61 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      121 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      181 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
      241 tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gctagatcta
      301 taacttcgta tagcatacat tatacgaagt tatgaattca ggaccttgaa cgcgaggtgc
      361 gcggctgtca ggattacgcg tgattatctg gcccgggcta ctcggtgtac aacaaggtct
      421 tcgatttcga ggcccctgtg cgcggtcaga atgccaaaat ggttatgacc tccgtatccg
      481 gccacatgat gcagctggca ttccaggtgt cttacaaaaa ttggagaacg gtggatccgc
      541 gctctttatt cgacgcgccg gtggaaaagg gcgtgggctc ggattatgag cctatcaaaa
      601 ggaccttgga acgcgaggtg cgcggctgtc agggattgat tatctggacg gattgcgatc
      661 gcgagggcga gaacattggc tacgagatta ttgatgtgcg gccgcatccg agccattgga
      721 agattaggtt ctgcatgcgc acacaattga tgacttgatc tagaataact tcgtatagca
      781 tacattatac gaagttatca tatgagctcc agcttttgtt ccctttagtg agggttaatt
      841 tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      901 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      961 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtc
//
