LOCUS       sequence-147891-        9957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, 
            Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song 
            JS, Ramalho-Santos M
  TITLE     YAP Induces Human Naive Pluripotency.
  JOURNAL   Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 
            10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3.
  PUBMED    26947063
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.02.036"; journalName: "Cell Rep"; date: 
            "2016-03-15- 15"; volume: "14"; issue: "10"; pages: "2301-12"
COMMENT     Sequence Label: pGAMA-empty
FEATURES             Location/Qualifiers
     source          1..9957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(96..115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(775..1566,1567..1635))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1398..1417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1636..1740)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1824..1843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2015..2394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2396..2594
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2549..2569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2612..2792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2839..2964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3463..3696
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3881..3925
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4223..4340
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4593..4626)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4630..4650
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4788..4999
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5000..5938
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5895..5915
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5954..6078
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6103..6108,6109..6126,6127..6133)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6187..6846
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6253..6272)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7188..7493
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7372..7391
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7534..7658)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7684..7725
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7729..7782
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      7789..7798
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7795..8505
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7940..7958)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8208..8227
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(8525..8558)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8614..9202
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8667..8687)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9085..9096)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9205..9221)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9206..9222)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9731..9911
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
       61 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
      841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
      901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
      961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1441 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1501 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1561 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1621 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1681 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1741 cacatttccc cgaaaagtgc cacctgacgc ttaagcggtc gacggatcgg gagatctccc
     1801 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     1861 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     1921 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     1981 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     2041 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     2101 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     2161 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     2221 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     2281 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     2341 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     2401 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     2461 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     2521 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     2581 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     2641 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2701 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2761 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     2821 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     2881 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     2941 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     3001 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     3061 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     3121 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     3181 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     3241 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     3301 gcggccggcc gcgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     3361 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     3421 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     3481 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     3541 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     3601 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     3661 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     3721 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     3781 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     3841 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     3901 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     3961 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     4021 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     4081 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     4141 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     4201 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     4261 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     4321 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     4381 taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg ccccctcgca
     4441 cagacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat ataatatttc
     4501 ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct ttttaatact
     4561 agctacattt tacatgatag gcttggattt ctataacttc gtatagcata cattatacga
     4621 agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg tgttttgaga
     4681 ctataagtat cccttggaga accaccttgt tggtttttct cgagtactag gatccattag
     4741 gcggccgcgt ggataaccgt attaccgcca tgcatgtgcc cgtcagtggg cagagcgcac
     4801 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     4861 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     4921 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     4981 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     5041 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     5101 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     5161 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     5221 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     5281 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     5341 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     5401 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     5461 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     5521 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     5581 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     5641 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     5701 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     5761 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     5821 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     5881 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     5941 gtcgtgagct agcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     6001 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     6061 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     6121 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     6181 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     6241 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     6301 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     6361 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     6421 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     6481 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     6541 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     6601 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     6661 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     6721 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     6781 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     6841 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     6901 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     6961 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     7021 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     7081 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     7141 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
     7201 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     7261 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     7321 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     7381 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     7441 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     7501 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     7561 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     7621 ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atctagaggg cccgcggttc
     7681 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtga gggcagagga
     7741 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caccggtcgc caccatggtg
     7801 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     7861 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     7921 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     7981 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     8041 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     8101 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     8161 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     8221 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     8281 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     8341 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     8401 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     8461 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagaatt cgtcgaggga
     8521 cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc
     8581 cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     8641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9001 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9121 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9181 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     9241 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     9301 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     9361 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     9421 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     9481 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     9541 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     9601 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     9661 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     9721 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9781 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9841 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9901 aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggc
//
