LOCUS       sequence-147902-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ATF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_ATF4
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..109)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(125..148)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(152..175)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(179..202)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(471..488)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
ORIGIN
        1 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccaggtccag
       61 ggttctcctc cacgtctcca gcctgcttca gcaggctgaa gttagtagct ccgcttcctg
      121 aaaccttatc gtcgtcatcc ttgtaatctc ccttatcgtc gtcatccttg taatcgccct
      181 tatcgtcgtc atccttgtaa tctccagaac cacctccacc ggaacctcct ccgctcccgg
      241 ggaccctttt cttccccctt gccttgcgga cctcttctat caaatctttc aggtactgga
      301 tctccttggc cagggaatcc gccctctctt ttagagcctc gttcttcttt tccagctctt
      361 tgcactcacc agtaagagcc tcctgctccg ccctcttctt ctggcggtac ctagtggctg
      421 ctgtcttgtt ttgctccatt tttttcagct tcttatccag tttctcaccc tttacttttg
      481 ctgctaccat cttctctcca ggaggatcgt aaggtttggg acgggcagac ccacagagaa
      541 cacctggaga tgggaggctc ctatttggag agcccctggt agaggggctg tgctgaggag
      601 accccagata ggactctggg ctcatacaga tgccactatc attatctgaa ggggtgtctt
      661 cctcctttat gcactgaggg atcatggcaa cgtaagcagt gtagtctggc ttcctatctc
      721 cttcagtgat atccacttca ctgcccagct ctaaactaaa ggaatgatct ggagtggagg
      781 acaggacccc tggggaaagg ggaagaggtt gtaagaaggt gaagggggca acctggtcgg
      841 gttttgttaa actttctggg agatggccaa ttgggttcac cgtctggggg ggctgcttat
      901 tagtctcctg gactaggggg gcaaagagat cacaagtgtc atccaaagct gggctgcagg
      961 tcggg
//
