LOCUS       sequence-147904-       10029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 
            1 protein MICU1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: MICU-APEX2
FEATURES             Location/Qualifiers
     source          1..10029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2101..2124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2158..2175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2176..2922
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(2927..2951)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2953..2994
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3036..3624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3089..3109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3507..3518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3696..3929
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4007..4128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4044..4063)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4098..4117
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4168..4303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4323..4342)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4324..4342)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4352..4369)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4352..4368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4361..4383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4510..4965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4597..4616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4807..4828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4991..5095
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5096..5164,5165..5956)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5314..5333)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6127..6715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6616..6635
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6869..6886
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7003..7024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7039..7044,7045..7062,7063..7069)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7077..7093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7082..7104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7101..7117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7101..7117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7136..7156
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7138..7156
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7184..7410
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7411..7591
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7638..7763
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8256..8489
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8674..8718
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8893..9393
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9312..9330
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9405..9800
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9860..9977
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgt ttcgtctgaa ctcactttct
      661 gctttggcag aactggctgt gggttctcga tggtaccatg gaggatcaca gcccatccag
      721 atccggcgaa gactaatgat ggtggctttc ctgggagcat ctgcagtaac tgcaagtact
      781 ggtcttttgt ggaagagggc ccatgcagaa tctccaccat gtgtagacaa cctaaaaagt
      841 gacatcggtg ataaagggaa gaataaagat gaaggggatg tttgtaacca tgagaaaaag
      901 actgcagatc ttgcccctca cccagaagag aaaaagaaga aacgttctgg attcagagac
      961 agaaaagtga tggaatatga gaataggatt cgagcctact ccacgccaga caaaatcttc
     1021 cgatattttg ccaccttgaa agtcatcagt gagcctggtg aagcagaagt gtttatgaca
     1081 ccagaagatt ttgtgcgatc cataacaccc aatgaaaaac aaccagaaca cttgggtctg
     1141 gatcaatata taataaaacg ctttgatgga aagaaaattt cccaggaacg agaaaaattt
     1201 gctgatgaag gcagtatatt ttacaccctt ggagaatgtg ggctcatatc cttttcagac
     1261 tacattttcc tcacaactgt tctttccact cctcagagaa attttgaaat tgccttcaag
     1321 atgtttgatt tgaatggaga tggagaagta gatatggaag aatttgaaca ggttcagagc
     1381 atcattcgct cccaaaccag tatgggtatg cgccacagag atcgtccaac tactggcaac
     1441 accctcaagt ctggcttgtg ttcagccctc acaacctact tttttggagc tgatctgaag
     1501 ggaaagctga caatcaaaaa cttcctcgaa tttcagcgta aactgcagca tgatgttctg
     1561 aagcttgagt ttgaacgcca tgaccctgtg gatgggagaa ttactgagag gcagtttggt
     1621 ggcatgctac ttgcctacag tggggtgcag tccaagaagc tgaccgccat gcagaggcag
     1681 ctcaagaagc acttcaaaga aggaaagggt ctgacatttc aggaggtgga gaacttcttt
     1741 actttcctaa agaacattaa tgatgtggac actgcattga gtttttacca tatggctgga
     1801 gcatctcttg ataaagtgac catgcagcag gtggccagga cagtggctaa agtggagctc
     1861 tcagaccacg tgtgtgatgt ggtgtttgca ctctttgact gtgatggaaa cggagagctc
     1921 agtaacaaag aatttgtttc catcatgaag caacggctga tgagaggcct ggaaaagccc
     1981 aaagacatgg gtttcactcg cctcatgcag gccatgtgga aatgtgcaca ggaaactgcc
     2041 tgggacttcg ctttacccaa acagcccggg ggtggatctg gtggatctgg tggatctatg
     2101 gattacaagg atgacgatga caagggtgga tctggtggat ctggtggatc tatggcacac
     2161 caccaccacc accacggaaa gtcttaccca actgtgagtg ctgattacca ggacgccgtt
     2221 gagaaggcga agaagaagct cagaggcttc atcgctgaga agagatgcgc tcctctaatg
     2281 ctccgtttgg cattccactc tgctggaacc tttgacaagg gcacgaagac cggtggaccc
     2341 ttcggaacca tcaagcaccc tgccgaactg gctcacagcg ctaacaacgg tcttgacatc
     2401 gctgttaggc ttttggagcc actcaaggcg gagttcccta ttttgagcta cgccgatttc
     2461 taccagttgg ctggcgttgt tgccgttgag gtcacgggtg gacctaaggt tccattccac
     2521 cctggaagag aggacaagcc tgagccacca ccagagggtc gcttgcccga tcccactaag
     2581 ggttctgacc atttgagaga tgtgtttggc aaagctatgg ggcttactga ccaagatatc
     2641 gttgctctat ctgggggtca cactattgga gctgcacaca aggagcgttc tggatttgag
     2701 ggtccctgga cctctaatcc tcttattttc gacaactcat acttcacgga gttgttgagt
     2761 ggtgagaagg aaggtctcct tcagctacct tctgacaagg ctcttttgtc tgaccctgta
     2821 ttccgccctc tcgttgacaa atatgcagcg gacgaagatg ccttctttgc tgattacgct
     2881 gaggctcacc aaaagctttc cgagcttggg tttgctgatg cctaagaccc agctttcttg
     2941 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     3001 tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat tacaaaattt
     3061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3601 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     3661 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3721 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     3781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     3841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3901 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     3961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     4021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     4141 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4201 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4261 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     4321 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     4381 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4441 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4501 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4921 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     4981 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5041 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5101 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5161 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5221 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5281 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5341 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5401 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5461 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5521 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5581 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5641 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5701 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5761 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5821 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5881 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5941 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6001 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6061 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6121 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6181 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6241 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6301 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6361 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6421 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6481 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6541 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6601 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6661 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6721 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6781 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6841 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6901 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6961 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7021 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7081 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     7141 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7201 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7261 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7321 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7381 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     7441 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7501 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7561 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7621 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7681 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7741 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7801 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7861 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7921 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7981 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8041 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8101 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8161 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8221 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8281 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8341 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8401 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8461 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8521 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8581 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8641 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8701 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     8761 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     8821 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     8881 ccgaggggac ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     8941 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     9001 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     9061 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     9121 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     9181 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     9241 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     9301 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     9361 ctcgttgacc gaatcaccga cctctctccc cagggggtac caccatggcc aagcctttgt
     9421 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     9481 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     9541 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     9601 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     9661 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     9721 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     9781 ctggttatgt gtgggagggc ctgcagctgc agtagtaaga attctagatc ttgagacaaa
     9841 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9901 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9961 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    10021 tcgaggggg
//
