LOCUS       sequence-147911-       11423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express rhesus TRIM5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: CSII-IDR2-rhesusTRIM5-FOS
FEATURES             Location/Qualifiers
     source          1..11423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2414..2531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2661..2964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2965..3168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4706..4729
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4736..4759
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4763..4815
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(4817..5391,5392..5394,5395..5403)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4984..5001)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5211..5230
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5404..6081
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(5584..5604)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5802..5821
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5996..6019
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6117..6705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6170..6190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6588..6599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7224..7404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7430..7447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7436..7660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7706..8134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7793..7812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8003..8024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8143..8163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8148..8478
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8329..8464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8391..8410
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8526..8573
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8592..8966
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9096..9217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9133..9152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9187..9206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9266..9282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9266..9282)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9279..9301)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9290..9306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9314..9320,9321..9338,9339..9344))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9359..9380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9497..9514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9668..10256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9748..9767)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10427..11218,11219..11287))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11050..11069
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11288..11392)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagatct ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcta caaatggcag
     2401 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2461 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2521 ttcaaaattt tcgggtttat tacagtgtac agaaacaccg tgagacgtca tgcggccgcg
     2581 cagaaattca ctttgaatta attcgattaa tagtaatcaa ttacggggtc attagttcat
     2641 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2701 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2761 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2821 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2881 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2941 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3001 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3061 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3121 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     3181 cgtcagatcc gctagcatgg cttctggaat cctgcttaat gtaaaggagg aggtgacctg
     3241 tcccatctgc ctggaactcc tgacagaacc cctgagtctg cactgcggcc acagcttctg
     3301 ccaagcgtgc atcactgcga accacaagaa gtccatgcta tacaaagaag gagagagaag
     3361 ctgccctgtg tgccggatca gttaccagcc tgagaacata cagcctaatc ggcatgtagc
     3421 caacatagtg gagaagctca gggaggtcaa gttgagccca gaagagggac agaaggttga
     3481 tcactgtgca cgccatggag agaaactcct actcttctgt caggaggaca gcaaggtcat
     3541 ttgctggctt tgtgagcggt ctcaggagca ccgtggtcac cacactttcc tcatggagga
     3601 ggttgcccag gagtaccatg tgaagctcca gacagctctg gagatgctga ggcagaagca
     3661 gcaggaagct gaaaagttgg aagctgacat cagagaagag aaagcttcct ggaagattca
     3721 aatagactac gacaaaacca acgtctcggc agattttgag caactgagag agatcctgga
     3781 ctgggaggag agcaatgagc tgcagaacct ggagaaggag gaagaagaca ttctgaaaag
     3841 ccttacgaag tctgaaacgg agatggtgca gcagacccag tacatgagag agctcatctc
     3901 agaactggag catcggttgc aggggtcaat gatggatcta ctgcagggtg tggatggcat
     3961 cattaaaagg attgagaaca tgaccttgaa gaagccaaaa acttttcaca aaaatcaaag
     4021 gagagtgttt cgagctcctg atctgaaagg aatgctagac atgtttagag agctaacaga
     4081 tgcccgacgc tactgggttg atgtgacact ggctacaaac aacatttcgc atgctgtcat
     4141 tgctgaagat aagagacaag tgagctctcg gaacccacag ataatgtatc aggcaccagg
     4201 gacattattt acgtttccgt cactcacgaa tttcaattat tgtactggca tcctgggctc
     4261 ccaaagtatc acatcaggga agcattactg ggaggtagat gtgtccaaga aaagtgcttg
     4321 gatcctgggg gtatgtgctg gcttccaatc cgatgcaatg tataatattg aacaaaatga
     4381 aaattatcaa cctaaatatg gctactgggt tatagggtta caggaaggag ttaaatatag
     4441 tgttttccag gatggttcct cacatactcc ttttgctcct ttcattgtgc ccctctctgt
     4501 gattatttgt cctgatcgtg ttggagtttt cgtagactat gaggcttgca ctgtctcatt
     4561 cttcaatatc acaaaccatg gatttctcat ctataagttt tctcagtgtt ctttttctaa
     4621 gcctgtattt ccatatttaa atcccagaaa atgtacagtc cccatgaccc tgtgctcacc
     4681 aagctctggt accggatccg gattcgatta caaggatgac gatgacaaga gcgcttggag
     4741 ccacccgcag ttcgaaaaat aactcgagct caagcttcga attctgcagt cgacggtacc
     4801 gcgggcccgg gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     4861 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     4921 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     4981 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5041 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5101 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5161 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5221 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5281 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5341 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     5401 accatggata gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt gcacatggag
     5461 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggcaa gccctacgag
     5521 ggcacccaga ccgccaagct gcaggtgacc aagggcggcc ccctgccctt cgcctgggac
     5581 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
     5641 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     5701 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg caccttcatc
     5761 taccacgtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag
     5821 actctgggct gggagccctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     5881 gagatccaca aggcgctgaa gctgaagggc ggcggccact acctggtgga gttcaagtca
     5941 atctacatgg ccaagaagcc cgtgaagctg cccggctact actacgtgga ctccaagctg
     6001 gacatcacct cccacaacga ggactacacc gtggtggagc agtacgagcg cgccgaggcc
     6061 cgccaccacc tgttccagta gtctagagtt aacatcgagg gatcaagctt atcgataatc
     6121 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6181 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6241 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6301 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6361 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6421 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6481 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     6541 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6601 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6661 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgag
     6721 acctggaaaa acatggagca atcacaagta gcaacacagc agctaccaat gctgcttgtg
     6781 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6901 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6961 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     7021 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     7081 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     7141 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     7201 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7381 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     7441 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7501 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7561 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7621 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7681 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7741 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7801 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7861 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7921 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7981 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8041 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
     8101 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     8161 ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat
     8221 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8281 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8401 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8461 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     8521 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     8581 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     8641 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     8701 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     8761 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     8821 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     8881 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     8941 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     9001 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     9061 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     9121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     9241 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9421 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9601 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9661 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9721 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9781 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9841 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     9901 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9961 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10021 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10081 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    10141 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10201 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10261 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10321 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10381 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10441 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10501 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10561 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10621 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10681 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10741 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10801 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10861 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10921 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10981 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11041 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11101 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11161 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11221 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11281 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11341 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11401 cccgaaaagt gccacctgac gtc
//
