LOCUS       sequence-147918-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express African green monkey pygerythrus TRIM5alpha in mammalan 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: CSII-IDR2-AGMpygTRIM5-FOS
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2414..2531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2661..2964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2965..3168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4760..4783
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4790..4813
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4817..4869
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(4871..5445,5446..5448,5449..5457)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5038..5055)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5265..5284
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5458..6135
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(5638..5658)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5856..5875
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6050..6073
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6171..6759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6224..6244)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6642..6653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7278..7458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7484..7501)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7490..7714
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7760..8188
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7847..7866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8057..8078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8197..8217)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8202..8532
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8383..8518
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8445..8464
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8580..8627
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8646..9020
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9150..9271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9187..9206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9241..9260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9320..9336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9320..9336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9333..9355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9344..9360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9368..9374,9375..9392,9393..9398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9413..9434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9551..9568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9722..10310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9802..9821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10481..11272,11273..11341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11104..11123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11342..11446)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagatct ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcta caaatggcag
     2401 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2461 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2521 ttcaaaattt tcgggtttat tacagtgtac agaaacaccg tgagacgtca tgcggccgcg
     2581 cagaaattca ctttgaatta attcgattaa tagtaatcaa ttacggggtc attagttcat
     2641 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2701 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2761 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2821 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2881 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2941 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3001 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3061 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3121 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     3181 cgtcagatcc gctagcatgg cttctggaat cctggttaat gtaaaggagg aggtgacctg
     3241 tcccatctgc ctggaactcc tgacagaacc cctgagtctg ccctgcggcc acagcctctg
     3301 ccaagcgtgc atcactgcaa accacaagga gtccatgcta tacaaagaag aagagagaag
     3361 ctgccctgtg tgccggatca gttaccagcc tgagaatata cagcctaatc ggcatgtggc
     3421 caacatagtg gagaagctca gagaggtcaa gttgagccca gaagaggggc agaaggttga
     3481 tcactgtgca cgccatggag agaaactcct actcttctgt caggaggaca gcaaggtcat
     3541 ttgctggctt tgtgagcggt ctcaggagca ccgtggtcac cacactttcc tcatggagga
     3601 ggttgcccag gagtaccatg tgaagctcca gacagctctg gagatgctga ggcagaagca
     3661 gcaggaagct gaaaagttgg aagctgacat cagagaagag aaagcttcct ggaagattca
     3721 aatagactac gacaaaacca acgtctcggc agattttgag caactgagag agatcctgga
     3781 ctgggaggag agcaatgagc tgcagaacct ggagaaggag gaagaagaca ttctgaaaag
     3841 ccttacgaag tctgaaacgg agatggtgca gcagacccag tacatgagag agctcatctc
     3901 agatctggag catcggttgc aggggtcaat gatggagctg ctgcagggtg tggatggcat
     3961 cattaaaagg gttgagaaca tgaccttgaa gaagccaaaa acatttcaca aaaatcaaag
     4021 gagagtgttt cgagctcctg atctgaaagg aatgctagac atgtttagag agctaacaga
     4081 tgtccgacgc tactgggttg atgtgacact ggctccaaac aacatttcgc atgctgtcat
     4141 tgctgaagat aagagacaag tgagctatcg gaacccacag ataatgtatc agtcaccagg
     4201 gtcattattt gggtcactca cgaatttcag ttattgtact ggcgtcccgg gctcccaaag
     4261 tatcacatca gggaaactca cgaatttcaa ttattgtact ggcgtcctgg gttcccaaag
     4321 tatcacatca gggaaacatt actgggaggt agatgtgtcc aagaaaagtg cttggatcct
     4381 gggggtatgt gctggcttcc aacccgatgc aacgtataat attgaacaaa atgaaaatta
     4441 tcaacctaaa tatggctact gggttatagg gttacaggaa ggagataaat atagtgcttt
     4501 ccaggatggt tcctcacata ctccttttgc tcctttcatt gtgcccctct ctgtgattat
     4561 ttgtcctgat cgtgttggag ttttcgtaga ctatgaggct tgcactgtct cattcttcaa
     4621 tatcacaaac catggatttc tcatctataa gttttctcag tgttcttttt ctaagcctgt
     4681 atttccatat ttaaatccca gaaaatgtac agtccccatg actctgtgct caccaagctc
     4741 tggtaccgga tccggattcg attacaagga tgacgatgac aagagcgctt ggagccaccc
     4801 gcagttcgaa aaataactcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     4861 ccgggatccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     4921 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     4981 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     5041 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     5101 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     5161 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     5221 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     5281 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     5341 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     5401 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     5461 gatagcactg agaacgtcat caagcccttc atgcgcttca aggtgcacat ggagggctcc
     5521 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gcaagcccta cgagggcacc
     5581 cagaccgcca agctgcaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     5641 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac
     5701 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     5761 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcacctt catctaccac
     5821 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa gaagactctg
     5881 ggctgggagc cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     5941 cacaaggcgc tgaagctgaa gggcggcggc cactacctgg tggagttcaa gtcaatctac
     6001 atggccaaga agcccgtgaa gctgcccggc tactactacg tggactccaa gctggacatc
     6061 acctcccaca acgaggacta caccgtggtg gagcagtacg agcgcgccga ggcccgccac
     6121 cacctgttcc agtagtctag agttaacatc gagggatcaa gcttatcgat aatcaacctc
     6181 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6241 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6301 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6361 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6421 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6481 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6541 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6601 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6661 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6721 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgagacctgg
     6781 aaaaacatgg agcaatcaca agtagcaaca cagcagctac caatgctgct tgtgcctggc
     6841 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6901 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6961 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     7021 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     7081 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     7141 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7201 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     7261 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7321 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7381 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7441 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     7501 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7561 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7621 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7681 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7741 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7801 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     7921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8101 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     8161 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     8221 gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca
     8281 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8341 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8401 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8461 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8521 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     8581 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     8641 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     8701 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     8761 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     8821 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     8881 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     8941 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     9001 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     9061 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9121 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     9181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     9301 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9901 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10141 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    10201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11461 aagtgccacc tgacgtc
//
