LOCUS       sequence-147921-       11411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express chimpanzee TRIM5alpha in mammalan cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 11411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: CSII-IDR2-chimpTRIM5-FOS
FEATURES             Location/Qualifiers
     source          1..11411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2414..2531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2661..2964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2965..3168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4694..4717
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4724..4747
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4751..4803
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(4805..5379,5380..5382,5383..5391)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4972..4989)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5199..5218
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5392..6069
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(5572..5592)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5790..5809
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5984..6007
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6105..6693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6158..6178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6576..6587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7212..7392
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7418..7435)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7424..7648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7694..8122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7781..7800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7991..8012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8131..8151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8136..8466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8317..8452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8379..8398
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8514..8561
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8580..8954
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9084..9205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9121..9140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9175..9194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9254..9270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9254..9270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9267..9289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9278..9294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9302..9308,9309..9326,9327..9332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9347..9368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9485..9502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9656..10244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9736..9755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10415..11206,11207..11275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11038..11057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11276..11380)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagatct ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcta caaatggcag
     2401 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2461 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2521 ttcaaaattt tcgggtttat tacagtgtac agaaacaccg tgagacgtca tgcggccgcg
     2581 cagaaattca ctttgaatta attcgattaa tagtaatcaa ttacggggtc attagttcat
     2641 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2701 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2761 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2821 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2881 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2941 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3001 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3061 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3121 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     3181 cgtcagatcc gctagcatgg cttctggaat cctggttaat gtaaaggagg aggtgacctg
     3241 ccccatctgc ctggaactcc tgacacaacc cctgagcctg gactgcggcc acagcttctg
     3301 ccaagcatgc ctcactgcaa accacaagaa gtccatgcta gacaaaggag agagtagctg
     3361 ccctgtgtgc cggatcagtt accagcctga gaacatacgg cctaatcggc atgcagccaa
     3421 catagtggag aagctcaggg aggtcaagtt gagcccagag gggcagaaag ttgatcattg
     3481 tgcacaccat ggagagaaac ttctactctt ctgtcaggag gacgggaagg tcatttgctg
     3541 gctttgtgag cggtctcagg agcaccgtgg tcaccacacg ttcctcacag aggaggttgc
     3601 ccgggagtac caagtgaagc tccaggcagc tctggagatg ctgaggcaga agcagcagga
     3661 agctgaagag ttggaagctg acatcagaga agagaaagct tcctggaaga ctcaaataca
     3721 gtatgacaaa accaacgtct tggcagattt tgagcaactg agagacatcc tggactggga
     3781 ggagagcaat gagctgcaaa acctggagaa ggaggaggaa gacattctga aaagccttac
     3841 gaagtctgaa actgagatgg tgcagcagac ccagtccgtg agagagctca tctcagatct
     3901 ggagcgtcgg ctgcaggggt cagtgatgga gctgcttcag ggtgtggatg gcgtcataaa
     3961 aaggatggag aacgtgacct tgaagaagcc agaaactttt ccaaaaaatc aaaggagagt
     4021 gtttcgagct cctgatctga aaggaatgct agaagtgttt agagagctga cagatgtccg
     4081 acgctactgg gttgatgtga cagtggctcc aaacaacatt tcatgtgctg tcatttctga
     4141 agatatgaga caagtgagct ctccgaaacc acagataata tatggggcac gagggacaag
     4201 atatcagaca tttatgaatt tcaattattg tactggcatc ctgggctctc aaagtatcac
     4261 atcagggaaa cattactggg aggtagacgt gtccaagaaa agtgcttgga tcctgggggt
     4321 atgtgctggc ttccaacctg atgcaatgtg taatattgaa aaaaatgaaa attatcaacc
     4381 taaatatggc tactgggtta tagggttaga ggaaggagtt aaatgtagtg ctttccagga
     4441 tggttccttc catactcctt ctgctccttt cattgtgccc ctctctgtga ttatttgtcc
     4501 tgatcgtgtt ggagttttcc tagactatga ggcttgcact gtctcattct tcaatatcac
     4561 aaaccatgga tctctcatct ataagttttc tcactgttct ttttctcagc ctgtatttcc
     4621 atatttaaat cctagaaaat gtggagtccc catgactctg tgctcaccaa gctctggtac
     4681 cggatccgga ttcgattaca aggatgacga tgacaagagc gcttggagcc acccgcagtt
     4741 cgaaaaataa ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
     4801 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4861 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4921 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4981 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5041 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5101 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5161 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5221 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5281 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5341 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggatagc
     5401 actgagaacg tcatcaagcc cttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     5461 ggccacgagt tcgagatcga gggcgagggc gagggcaagc cctacgaggg cacccagacc
     5521 gccaagctgc aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     5581 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
     5641 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     5701 gtggtgaccg tgacccagga ctcctccctg caggacggca ccttcatcta ccacgtgaag
     5761 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac tctgggctgg
     5821 gagccctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag
     5881 gcgctgaagc tgaagggcgg cggccactac ctggtggagt tcaagtcaat ctacatggcc
     5941 aagaagcccg tgaagctgcc cggctactac tacgtggact ccaagctgga catcacctcc
     6001 cacaacgagg actacaccgt ggtggagcag tacgagcgcg ccgaggcccg ccaccacctg
     6061 ttccagtagt ctagagttaa catcgaggga tcaagcttat cgataatcaa cctctggatt
     6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6661 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgagac ctggaaaaac
     6721 atggagcaat cacaagtagc aacacagcag ctaccaatgc tgcttgtgcc tggctagaag
     6781 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6841 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6901 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6961 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7021 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7081 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7141 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7201 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7261 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7321 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7381 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     7441 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7501 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7561 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7621 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7681 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     7861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8041 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     8101 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8161 ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8221 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8281 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8341 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8401 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8461 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     8521 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     8581 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     8641 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     8701 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     8761 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     8821 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     8881 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     8941 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     9001 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     9061 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     9121 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9181 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     9241 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9301 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9361 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9421 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9841 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10081 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10261 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10321 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    10381 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    10441 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    10501 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    10561 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10621 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10681 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10741 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10801 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10861 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10921 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10981 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11041 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11101 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11161 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    11221 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    11281 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    11341 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    11401 cacctgacgt c
//
