LOCUS       sequence-147923-       11354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express owl monkey TRIMCyp in mammalan cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11354)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 11354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: CSII-IDR2-owlTRIMCyp-FOS
FEATURES             Location/Qualifiers
     source          1..11354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2414..2531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2661..2964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2965..3168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4637..4660
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4667..4690
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4694..4746
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(4748..5322,5323..5325,5326..5334)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4915..4932)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5142..5161
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5335..6012
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(5515..5535)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5733..5752
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5927..5950
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6048..6636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6101..6121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6519..6530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7155..7335
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7361..7378)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7367..7591
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7637..8065
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7724..7743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7934..7955
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8074..8094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8079..8409
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8260..8395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8322..8341
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8457..8504
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8523..8897
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9027..9148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9064..9083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9118..9137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9197..9213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9197..9213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9210..9232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9221..9237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9245..9251,9252..9269,9270..9275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9290..9311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9428..9445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9599..10187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9679..9698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10358..11149,11150..11218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10981..11000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11219..11323)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagatct ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcta caaatggcag
     2401 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2461 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2521 ttcaaaattt tcgggtttat tacagtgtac agaaacaccg tgagacgtca tgcggccgcg
     2581 cagaaattca ctttgaatta attcgattaa tagtaatcaa ttacggggtc attagttcat
     2641 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2701 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2761 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2821 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2881 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2941 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3001 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3061 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3121 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     3181 cgtcagatcc gctagcatgg cttccagaat cctggtcaat ataaaggagg aggtgacctg
     3241 ccccatctgc ctggaactcc tgacagaacc cctgagcctg gactgtggcc atagcttctg
     3301 ccaagcatgc atcactgcaa atcacaaaaa gtctatgcca caccaaggag agagaagctg
     3361 ccctttgtgc cggatcagtt actcgtctga gaacctgcgg cctaatcggc atttggtcaa
     3421 catagtggag aggctcaggg aggtcatgct gagcccagag gaggggcaga aggttgatca
     3481 ctgtgcacac catggagaga aacttgtact cttctgtcag caggatggaa atgtcatttg
     3541 ctggctttgt gagcggtctc aagaacaccg tgggcaccag acattccttg tggaggaggt
     3601 tgcacagaaa taccgagaaa agctccaggt agctctggag atgatgaggc agaagcagaa
     3661 ggatgctgaa aagttggaag ctgacgtcag agaagagcaa gcttcctgga agattcaaat
     3721 acaaaatgac aaaaccaaca tcatggcaga gtttaaaaaa cggagagaca tcctggactg
     3781 tgaggagagc aaagagttgc aaaacctgga gaaggaggag aaaaacattc tgaaaagact
     3841 tgtacagtct gaaaatgaca tggtgctgca gacccagtcc gtgagagtgc tcatctcaga
     3901 tctggagcat cgcctgcagg ggtcagtgat ggagctgtta cagggtgtgg atggtgtcat
     3961 aaaaaggatt gagaaagtga ctttgcagaa tccaaaaacc tttcttaatg aaaaaaggag
     4021 aatatttcaa actcctgatc tgaaaggaac actacaagtg tttaaagagc cgacagaagt
     4081 ccaacgctac tgggacgccg ccgcctggga ccttgtagca tcagccatgg tcaatcctac
     4141 tgtgttcttc gacattgccg tcgatggcga gcccttgggc cgcgtctcct tcgagctgtt
     4201 tgcagacaag gttccaaaga cagcagaaaa ctttcgtgct ctgagcactg gagagaaagg
     4261 atttggttat aagggttcct gctttcacag aattattcca gggtttatgt gtcagggtgg
     4321 tgacttcaca cgccataatg gcactggtgg caagtccatc tacggggaga aatttgatga
     4381 tgagaacttc atcctaaagc atacaggtcc cggtatcttg tccatggcaa atgctggacc
     4441 caacacaaac ggttcccagt ttttcatctg cactgccaag actgagtggt tggatggcaa
     4501 gcatgtggtc tttggcaagg tgaaagaagg catgaatgtt gtggaggcca tggagcgctt
     4561 tgggtgcagg tatggcaaga ccagcaaaaa gatcaccatt gctgactgtg gacaacttgg
     4621 taccggatcc ggattcgatt acaaggatga cgatgacaag agcgcttgga gccacccgca
     4681 gttcgaaaaa taactcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
     4741 ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     4801 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     4861 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     4921 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     4981 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5041 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5101 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5161 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5221 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5281 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggat
     5341 agcactgaga acgtcatcaa gcccttcatg cgcttcaagg tgcacatgga gggctccgtg
     5401 aacggccacg agttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag
     5461 accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     5521 ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac
     5581 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5641 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcaccttcat ctaccacgtg
     5701 aagttcatcg gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gactctgggc
     5761 tgggagccct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     5821 aaggcgctga agctgaaggg cggcggccac tacctggtgg agttcaagtc aatctacatg
     5881 gccaagaagc ccgtgaagct gcccggctac tactacgtgg actccaagct ggacatcacc
     5941 tcccacaacg aggactacac cgtggtggag cagtacgagc gcgccgaggc ccgccaccac
     6001 ctgttccagt agtctagagt taacatcgag ggatcaagct tatcgataat caacctctgg
     6061 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6121 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6181 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6241 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6301 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6361 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6421 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6481 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6541 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6601 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga gacctggaaa
     6661 aacatggagc aatcacaagt agcaacacag cagctaccaa tgctgcttgt gcctggctag
     6721 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6781 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6841 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6901 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     6961 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     7021 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7081 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     7141 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7201 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7261 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7321 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     7381 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7441 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7501 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7561 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     7621 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7681 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7741 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct
     7801 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7861 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7921 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7981 tattcttttg atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg
     8041 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     8101 agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8161 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8221 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8281 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8341 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8401 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     8461 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     8521 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     8581 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     8641 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     8701 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     8761 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     8821 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     8881 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     8941 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     9001 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     9061 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9121 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     9181 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9241 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9301 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9361 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9421 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9481 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9541 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9601 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9661 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9721 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9781 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9841 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9901 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9961 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10021 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10081 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10141 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10201 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10261 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10321 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10381 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10441 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10501 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10561 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10621 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10681 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10741 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10801 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10861 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10921 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10981 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11041 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11101 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11161 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11221 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11281 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11341 tgccacctga cgtc
//
