LOCUS       sequence-147929-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin
            promoter, in floxed/reversed (Cre-dependent) manner..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, 
            Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, 
            Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y,
            Tye KM, Roska B, Cardin JA, Boyden ES
  TITLE     Noninvasive optical inhibition with a red-shifted microbial 
            rhodopsin.
  JOURNAL   Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014
            Jul 6.
  PUBMED    24997763
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3752"; 
            journalName: "Nat Neurosci"; date: "2014-08"; volume: "17"; issue: 
            "8"; pages: "1123-9"
COMMENT     Sequence Label: pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..124
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    195..228
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattat
       61 cgataagctt cgcgaacctc ttcgagggac ataacttcgt ataatgtatg ctatacgaag
      121 ttatgggtcg atggtgagat ctggactaga gggtcgatgg tgatgcttgg atcaaaatag
      181 gaagaatccc aatgataact tcgtataaag tatcctatac gaagttatac taggtaccta
      241 ggccgcgccg ccaccatgac cgccgtgagc accacagcca ctaccgtgct gcaggccaca
      301 cagagcgacg tgctgcagga gatccagtcc aacttcctgc tgaatagctc catctgggtg
      361 aacattgctc tggccggagt ggtcatcctg ctgtttgtgg ccatggggag ggatctggaa
      421 tcccctagag ctaagctgat ctgggtggcc acaatgctgg tgccactggt gtctatttct
      481 agttacgctg gactggccag tgggctgact gtgggcttcc tgcagatgcc acctggacac
      541 gctctggccg gacaggaggt gctgagccca tggggccggt atctgacatg gactttctcc
      601 actcccatga tcctgctggc tctgggactg ctggccgaca ccgatattgc cagcctgttc
      661 accgccatca caatggacat tggcatgtgc gtgacaggac tggccgctgc cctgatcact
      721 agctcccatc tgctgcgctg ggtgttctac ggaatttctt gtgctttctt tgtggcc
//
