LOCUS       sequence-147931-        2900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing mIFP and EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: pLenti CMV_mIFP IRES EGFP
FEATURES             Location/Qualifiers
     source          1..2900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..960
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             964..975
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             967..1020
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2181..2183,2184..2186,2187..2900)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2226..2247)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2487..2506)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2834..2855
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtcggtac cgctgactac ctcagcattc ggccacgcgt ttctggctaa ctgtgaacgc
       61 gagcagatcc acctggcggg ctccattcag ccgcacggta tcctgctggc tgtgaaagag
      121 ccggacaacg tggtgatcca ggcttctatt aacgctgcgg agttcctgaa caccaactct
      181 gttgttggcc gtccgctgcg tgacctgggc ggcgatctgc ctttgcagat cctgccgcac
      241 ctgaacggcc cgctgcacct ggctccgatg accctgcgtt gtaccgtggg ttctccgccg
      301 cgtcgtgtgg actgtaccat tcatcgtccg tctaacggcg gcctgatcgt agaactggaa
      361 ccagcaacca agaccactaa cattgcgccg gctctggacg gtgcgtttca tcgtatcact
      421 tcttcatcct ccctgatggg cctgtgtgac gaaaccgcga ctattatccg tgagattact
      481 ggctacgacc gtgtgatggt agtacgtttc gatgaagagg gtaatggcga aattctgtcc
      541 gaacgtcgtc gtgcggacct ggaagcgttc ctgggtaacc gctacccggc gtctactatt
      601 ccgcagatcg ctcgtcgcct gtacgaacat aaccgtgttc gcctgctggt agatgtgaac
      661 tatactccgg ttccgctaca gccgcgcatc agcccgctga acggtcgtga tctggatatg
      721 tccctgtctt gcctgcgctc tatgtccccg atccaccaga aatacatgca ggacatgggc
      781 gttggcgcga ccctggtttg ctctctgatg gtgtctggtc gtctgtgggg tctgatcgct
      841 tgccaccact acgaaccgcg cttcgttccg ttccacattc gcgctgctgg cgaagcgctg
      901 gcggaaactt gtgcgatccg catcgcgacg ctggagagct ttgcacagtc tcagtccaaa
      961 gatatcgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     1021 atgaatatcg aagagttctt cgagctgagt gccgggaagt ggttttccca ccggacttcc
     1081 caccacctgg cttttaagca gtccgaagac ggcaagtctg atctggtgat agagagtctg
     1141 gccgcagatc accccgaggt gatcaaactc tgtgagctgt acgaggtgcc tgcttcagcc
     1201 gcaagttgcg gcgcaagagt ttcctggaac ggcaccatgg aatgggatga ggagaaacat
     1261 acagggtcca ccgtgctggc taccgtgccc gacgtggata accccaacga aggcaggctc
     1321 ctgcgcgaga tgggttacgc tgagaaagct cccgtggcag gccgctacaa aatgggcgac
     1381 gatggagcac tgactctgac cacagaatac gagaccatgt ggtcagaaga acggctgtgg
     1441 ttcgcaagcc ccaacctccg catgcgggtc tccgttctca agaggttcgg cgggttctca
     1501 atggccagct ttaccagtga gatacggatg ggaggatccc cagccgcagc caaagcagag
     1561 gaagccgcca atagcgcatc ctcatgactc gagtactccg gtattgcggt acccttgtac
     1621 gcctgtttta tactcccttc ccgtaactta gacgcacaaa accaagttca atagaagggg
     1681 gtacaaacca gtaccaccac gaacaagcac ttctgtttcc ccggtgatgt cgtatagact
     1741 gcttgcgtgg ttgaaagcga cggatccgtt atccgcttat gtacttcgag aagcccagta
     1801 ccacctcgga atcttcgatg cgttgcgctc agcactcaac cccagagtgt agcttaggct
     1861 gatgagtctg gacatccctc accggtgacg gtggtccagg ctgcgttggc ggcctaccta
     1921 tggctaacgc catgggacgc tagttgtgaa caaggtgtga agagcctatt gagctacata
     1981 agaatcctcc ggcccctgaa tgcggctaat cccaacctcg gagcaggtgg tcacaaacca
     2041 gtgattggcc tgtcgtaacg cgcaagtccg tggcggaacc gactactttg ggtgtccgtg
     2101 tttcctttta ttttattgtg gctgcttatg gtgacaatca cagattgtta tcataaagcg
     2161 aattggatag gatccgtacg atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2221 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2281 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2341 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2401 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2461 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2521 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2581 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2641 tggccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     2701 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2761 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2821 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2881 tggacgagct gtacaagtaa
//
