LOCUS       sequence-147936-        1740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing of iBlueberry and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: iBlueberry T2A mCherry
FEATURES             Location/Qualifiers
     source          1..1740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..960
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGQILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPCHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             967..1020
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1027..1740
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGA
                     LKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYE
                     RAEGRHSTGGMDELYK"
     primer_bind     complement(1178..1196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1446..1465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgtcggtac cgctgactac ctcagcattc ggccacgcgt ttctggctaa ctgtgaacgc
       61 gagcagatcc acctggcggg ctccattcag ccgcacggta tcctgctggc tgtgaaagag
      121 ccggacaacg tggtgatcca ggcttctatt aacgctgcgg agttcctgaa caccaactct
      181 gttgttggcc gtccgctgcg tgacctgggc ggcgatctgc ctttgcagat cctgccgcac
      241 ctgaacggcc cgctgcacct ggctccgatg accctgcgtt gtaccgtggg ttctccgccg
      301 cgtcgtgtgg actgtaccat tcatcgtccg tctaacggcg gcctgatcgt agaactggaa
      361 ccagcaacca agaccactaa cattgcgccg gctctggacg gtgcgtttca tcgtatcact
      421 tcttcatcct ccctgatggg cctgtgtgac gaaaccgcga ctattatccg tgagattact
      481 ggctacgacc gtgtgatggt agtacgtttc gatgaagagg gtaatggcca aattctgtcc
      541 gaacgtcgtc gtgcggacct ggaagcgttc ctgggtaacc gctacccggc gtctactatt
      601 ccgcagatcg ctcgtcgcct gtacgaacat aaccgtgttc gcctgctggt agatgtgaac
      661 tatactccgg ttccgctaca gccgcgcatc agcccgctga acggtcgtga tctggatatg
      721 tccctgtctt gcctgcgctc tatgtccccg tgccaccaga aatacatgca ggacatgggc
      781 gttggcgcga ccctggtttg ctctctgatg gtgtctggtc gtctgtgggg tctgatcgct
      841 tgccaccact acgaaccgcg cttcgttccg ttccacattc gcgctgctgg cgaagcgctg
      901 gcggaaactt gtgcgatccg catcgcgacg ctggagagct ttgcacagtc tcagtccaaa
      961 ggatccgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     1021 gaattcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     1081 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     1141 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     1201 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     1261 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     1321 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     1381 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1441 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1501 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1561 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1621 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1681 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
//
