LOCUS       sequence-147937-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An adaption on the RCAS/tv-a somatic cell gene transfer system, for 
            use in combination with an existing Cas9 background in the 
            cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Fraser J, Simpson J, Fontana R, Kishi-Itakura C, Ktistakis NT, 
            Gammoh N
  TITLE     Targeting of early endosomes by autophagy facilitates EGFR recycling
            and signalling.
  JOURNAL   EMBO Rep. 2019 Oct 4;20(10):e47734. doi: 10.15252/embr.201947734. 
            Epub 2019 Aug 26.
  PUBMED    31448519
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2019-10-4"; volume: "20(10):e47734. doi"; pages: " 
            10.15252/embr.201947734. Epub 2019 Aug 26"
COMMENT     Sequence Label: RCAS-sgATG7
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        180..428
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     180..200
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     351..370
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        449..524
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(536..560)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        575..789
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     710..729
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 gcaggaaaga cagctattgg taattgtgaa atacgctttt gtctgtgtgc tgcaggagct
       61 gagctgactc tgctggtggc ctcgcgtacc actgtggcat cggcggccgc tgtacaaaaa
      121 agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc gggcaggaag
      181 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      241 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      301 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      361 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      421 gaaacaccgg aaacttgttg aggagcatgt tttagagcta gaaatagcaa gttaaaataa
      481 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tctagaccca
      541 gctttcttgt acaaagttgg cattagcggc cgccgaacgc tgacgtcatc aacccgctcc
      601 aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag
      661 gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt cgctatgtgt
      721 tctgggaaat caccataaac gtgaaatgtc tttggatttg ggaatcttat aagttctgta
      781 tgagaccaca gatcccc
//
