LOCUS       sequence-147939-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the double Holliday junction 
            substrate (DHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Plank JL, Hsieh TS
  TITLE     A novel, topologically constrained DNA molecule containing a double 
            Holliday junction: design, synthesis, and initial biochemical 
            characterization.
  JOURNAL   J Biol Chem. 2006 Jun 23;281(25):17510-6. Epub 2006 Apr 11.
  PUBMED    16608853
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-06-23"; volume: "281(25)"; pages: "17510-6. Epub
            2006 Apr 11"
COMMENT     Sequence Label: pDHJS3 BS-
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     236..258
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     250..267
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     251..267
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     274..293
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        274..292
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     317..333
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     318..334
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(348..381)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(649..668)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    complement(764..797)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(821..839)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(860..876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(860..876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(873..895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    884..900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(908..914,915..932,933..938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    953..974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
       61 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca
      121 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      181 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      241 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
      301 ggtaccgggc cccccctcga ggtcgacggt atcgataagc tagatctata acttcgtata
      361 gcatacatta tacgaagtta tgaattcagg accttgaacg cgaggtgcgc ggctgtcagg
      421 attacgcgtg attatctggc ccggggctat atgcgttgat gcaatttcta tgcgcacccg
      481 ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
      541 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg
      601 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      661 acatcaccga tggggaagat cgggctcgcc acttcggcgg ccgcatccga gccattggaa
      721 gattaggttc tgcatgcgca cacaattgat gacttgatct agaataactt cgtatagcat
      781 acattatacg aagttatcat atgagctcca gcttttgttc cctttagtga gggttaattt
      841 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      901 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      961 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacc
//
