LOCUS       sequence-147943-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the mismatched double Holliday
            junction substrate (MM-DHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Plank JL, Hsieh TS
  TITLE     A novel, topologically constrained DNA molecule containing a double 
            Holliday junction: design, synthesis, and initial biochemical 
            characterization.
  JOURNAL   J Biol Chem. 2006 Jun 23;281(25):17510-6. Epub 2006 Apr 11.
  PUBMED    16608853
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-06-23"; volume: "281(25)"; pages: "17510-6. Epub
            2006 Apr 11"
COMMENT     Sequence Label: pDHJS3 AN-
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     234..256
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     248..265
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     249..265
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     272..291
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        272..290
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     315..331
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     316..332
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(346..379)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(810..843)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(867..885)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(906..922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(906..922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(919..941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    930..946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(954..960,961..978,979..984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
       61 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
      121 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      181 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      241 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg
      301 taccgggccc cccctcgagg tcgacggtat cgataagcta gatctataac ttcgtatagc
      361 atacattata cgaagttatg aattcaggac cttgaacgcg aggtgcgcgg ctgtcaggat
      421 tacgcgtgat tatctggccg ggctactcgg tgtacaacaa ggtcttcgat ttcgaggccc
      481 ctgtgcgcgg tcagaatgcc aaaatggtta tgacctccgt atccggccac atgatgcagc
      541 tggcattcca ggtgtcttac aaaaattgga gaacggtgga tccgcgctct ttattcgacg
      601 cgccggtgga aaagggcgtg ggctcggatt atgagcctat caaaaggacc ttggaacgcg
      661 aggtgcgcgg ctgtcaggga ttgattatct ggacggattg cgatcgcgag ggcgagaaca
      721 ttggctacga gattattgat gtgcggccgc atccgagcca ttggaagatt aggttctgct
      781 agcgcacaca attgatgact tgatctagaa taacttcgta tagcatacat tatacgaagt
      841 tatcatatga gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
      901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      961 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagc
//
