LOCUS       sequence-147946-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline inducible Ngn3 over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iNgn3
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(692..716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaccaattca gtcgactgga tccatggcgc ctcatccctt ggatgcgctc accatccaag
       61 tgtccccaga gacacaacaa ccttttcccg gagcctcgga ccacgaagtg ctcagttcca
      121 attccacccc acctagcccc actctcatac ctagggactg ctccgaagca gaagtgggtg
      181 actgccgagg gacctcgagg aagctccgcg cccgacgcgg agggcgcaac aggcccaaga
      241 gcgagttggc actcagcaaa cagcgaagaa gccggcgcaa gaaggccaat gatcgggagc
      301 gcaatcgcat gcacaacctc aactcggcgc tggatgcgct gcgcggtgtc ctgcccacct
      361 tcccggatga cgccaaactt acaaagatcg agaccctgcg cttcgcccac aactacatct
      421 gggcactgac tcagacgctg cgcatagcgg accacagctt ctatggcccg gagccccctg
      481 tgccctgtgg agagctgggg agccccggag gtggctccaa cggggactgg ggctctatct
      541 actccccagt ctcccaagcg ggtaacctga gccccacggc ctcattggag gaattccctg
      601 gcctgcaggt gcccagctcc ccatcctatc tgctcccggg agcactggtg ttctcagact
      661 tcttgtgagc ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat
      721 ccggggatcc tctagtcagc tgacgcgtgc tagcgcggcc gcatcgataa gcttgtcgac
      781 gatatctcta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      841 aaa
//
