LOCUS       sequence-147948-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline inducible Notch1 intracellular domain gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iNotch1IC
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaattccgat caacaagttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
       61 cggtatcgat aagcttgata attctagcta gctagccggc ccaattcggt accatggact
      121 acaaagacga tgacgataaa gcccggcggc agcatggcca gctctggttc cctgagggtt
      181 tcaaagtgtc agaggccagc aagaagaagc ggagagagcc cctcggcgag gactcagtcg
      241 gcctcaagcc cctgaagaat gcctcagatg gtgctctgat ggacgacaat cagaacgagt
      301 ggggagacga agacctggag accaagaagt tccggtttga ggagccagta gttctccctg
      361 acctgagtga tcagactgac cacagacagt ggacccagca gcacctggac gctgctgacc
      421 tgcgcatgtc tgccatggcc ccaacaccgc ctcaggggga ggtggatgct gactgcatgg
      481 atgtcaatgt tcgaggacca gatggcttca cacccctcat gattgcctcc tgcagtggag
      541 ggggccttga gacaggcaac agtgaagaag aagaagatgc acctgctgtc atctctgact
      601 tcatctacca gggcgccagc ttgcacaacc agacagaccg caccggggag accgccttgc
      661 acttggctgc ccgatactct cgttcagatg ctgcaaagcg cttgctggag gccagtgcag
      721 atgccaacat ccaggacaac atgggccgta ctccgttaca tgcagcagtt tctgcagatg
      781 ctcagggtgt cttccagatc ctgctccgga acagggccac agatctggat gcccgaatgc
      841 atgatggcac aactccactg atcctggctg cgcgcctggc cctggagggc atgctggagg
      901 acctcatcaa ctcacatgct gacgtcaatg ccgtggatga cctaggcaag tcggctttgc
      961 attgggcggc cgcggtgaac aatgtgga
//
