LOCUS       sequence-147949-         542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline inducible Notch1 intracellular domain gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iNotch1IC
FEATURES             Location/Qualifiers
     source          1..542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    202..226
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatcctctag agatatcgtc gacaagctta tcgatgcggc cgcgctagca cgcgtcagct
      181 gactagagga tccccggatc aaccactttg tacaagaaag ctgggtctag atatctcgag
      241 tgcggccgcg aattcgataa ttctcgagct agctacctag cttgccggtg cacccacggt
      301 gaaattcata gcccccgtgc cattggtact cagcactgtg ctggcactgg aggctacagg
      361 caggtgggag aggcgtggcg ggggtggctc aaaggccaac cggctacctc cagccagtgc
      421 tgccatctca ggcttggctg ccacattcaa gtggctgatg cccaggtgag tgtcaggcat
      481 accaggcagg tggctgagag gcatggatgg agactgctgg aatggggagg ggaggagggg
      541 tg
//
