LOCUS       sequence-147952-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline inducible NGN3 gene over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Hygro-iNGN3
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(743..767)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(966..985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gaattccgat caacaagttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
       61 ctggatccgc caccatgacg cctcaaccct cgggtgcgcc cactgtccaa gtgacccgtg
      121 agacggagcg gtccttcccc agagcctcgg aagacgaagt gacctgcccc acgtccgccc
      181 cgcccagccc cactcgcaca cgggggaact gcgcagaggc ggaagaggga ggctgccgag
      241 gggccccgag gaagctccgg gcacggcgcg ggggacgcag ccggcctaag agcgagttgg
      301 cactgagcaa gcagcgacgg agtcggcgaa agaaggccaa cgaccgcgag cgcaatcgaa
      361 tgcacaacct caactcggca ctggacgccc tgcgcggtgt cctgcccacc ttcccagacg
      421 acgcgaagct caccaagatc gagacgctgc gcttcgccca caactacatc tgggcgctga
      481 ctcaaacgct gcgcatagcg gaccacagct tgtacgcgct ggagccgccg gcgccgcact
      541 gcggggagct gggcagccca ggcggttccc ccggggactg ggggtccctc tactccccag
      601 tctcccaggc tggcagcctg agtcccgccg cgtcgctgga ggagcgaccc gggctgctgg
      661 gggccacctc ttccgcctgc ttgagcccag gcagtctggc tttctcagat tttctgtgag
      721 cggccgcact cgagatatct agacccagct ttcttgtaca aagtggttga tccggggatc
      781 ctctagtcag cgtttatctg cagaattcgc ccttgtttaa acgatcataa tcagccatac
      841 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      901 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttt
//
