LOCUS       sequence-147953-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline inducible NGN3 gene over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Hygro-iNGN3
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..162)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    186..211
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    218..1021
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(1035..1054)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1036..1054)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
       61 cttctcgaca gacgtcgcgg tgagttcagg ctttttcatc tgcagcctag ggccgggatt
      121 ctcctccacg tcaccgcatg ttagaagact tcctctgccc tctccgctgc cagatctctc
      181 gaggccctgt gggaggaaga gaagaggtca gggatctgcc ccactgtggg gtggagggga
      241 cagataaaag tacccagaac cagagccaca ttaaccggcc ctgggaatat aaggtggtcc
      301 cagctcgggg acacaggatc cctggaggca gcaaacatgc tgtcctgaag tggacatagg
      361 ggcccgggtt ggaggaagaa gactagctga gctctcggac ccctggaaga tgccatgaca
      421 gggggctgga agagctagca cagactagag aggtaagggg ggtaggggag ctgcccaaat
      481 gaaaggagtg agaggtgacc cgaatccaca ggagaacggg gtgtccaggc aaagaaagca
      541 agaggatgga gaggtggcta aagccaggga gacggggtac tttggggttg tccagaaaaa
      601 cggtgatgat gcaggcctac aagaagggga ggcgggacgc aagggagaca tccgtcggag
      661 aaggccatcc taagaaacga gagatggcac aggccccaga aggagaagga aaagggaacc
      721 cagcgagtga agacggcatg gggttgggtg agggaggaga gatgcccgga gaggacccag
      781 acacggggag gatccgctca gaggacatca cgtggtgcag cgccgagaag gaagtgctcc
      841 ggaaagagca tccttgggca gcaacacagc agagagcaag gggaagaggg agtggaggaa
      901 gacggaacct gaaggaggcg gcagggaagg atctgggcca gccgtagagg tgacccaggc
      961 cacaagctgc agacagaaag cggcacaggc ccaggggaga gaatgctggt cagagaaagc
     1021 aggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttt
//
