LOCUS       sequence-147955-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY destination vector for site-specific integration into the 
            human AAVS1/PPP1R12C locus for doxycycline-inducible gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iDEST
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..38
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    73..876
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 atgattacgc caagctcaga attaaccctc actaaaggga ctagtcctgc aggtttaaac
       61 gaattcgccc tttgctttct ctgaccagca ttctctcccc tgggcctgtg ccgctttctg
      121 tctgcagctt gtggcctggg tcacctctac ggctggccca gatccttccc tgccgcctcc
      181 ttcaggttcc gtcttcctcc actccctctt ccccttgctc tctgctgtgt tgctgcccaa
      241 ggatgctctt tccggagcac ttccttctcg gcgctgcacc acgtgatgtc ctctgagcgg
      301 atcctccccg tgtctgggtc ctctccgggc atctctcctc cctcacccaa ccccatgccg
      361 tcttcactcg ctgggttccc ttttccttct ccttctgggg cctgtgccat ctctcgtttc
      421 ttaggatggc cttctccgac ggatgtctcc cttgcgtccc gcctcccctt cttgtaggcc
      481 tgcatcatca ccgtttttct ggacaacccc aaagtacccc gtctccctgg ctttagccac
      541 ctctccatcc tcttgctttc tttgcctgga caccccgttc tcctgtggat tcgggtcacc
      601 tctcactcct ttcatttggg cagctcccct acccccctta cctctctagt ctgtgctagc
      661 tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta gtcttcttcc
      721 tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc agggatcctg
      781 tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct ctggttctgg
      841 gtacttttat ctgtcccctc caccccacag tggggcaagc ttctgacctc ttctcttcct
      901 cc
//
