LOCUS       sequence-147956-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY destination vector for site-specific integration into the 
            human AAVS1/PPP1R12C locus for doxycycline-inducible gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iDEST
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    55..891
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    916..934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    952..970
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    987..1005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgaa tttagcggcc gcgaattcgc ccttagagca
       61 gagccaggaa cccctgtagg gaaggggcag gagagccagg ggcatgagat ggtggacgag
      121 gaagggggac agggaagcct gagcgcctct cctgggcttg ccaaggactc aaacccagaa
      181 gcccagagca gggccttagg gaagcgggac cctgctctgg gcggaggaat atgtcccaga
      241 tagcactggg gactctttaa ggaaagaagg atggagaaag agaaagggag tagaggcggc
      301 cacgacctgg tgaacaccta ggacgcacca ttctcacaaa gggagttttc cacacggaca
      361 cccccctcct caccacagcc ctgccaggac ggggctggct actggcctta tctcacaggt
      421 aaaactgacg cacggaggaa caatataaat tggggactag aaaggtgaag agccaaagtt
      481 agaactcagg accaacttat tctgattttg tttttccaaa ctgcttctcc tcttgggaag
      541 tgtaaggaag ctgcagcacc aggatcagtg aaacgcacca gacagccgcg tcagagcagc
      601 tcaggttctg ggagagggta gcgcagggtg gccactgaga accgggcagg tcacgcatcc
      661 cccccttccc tcccaccccc tgccaagctc tccctcccag gatcctctct ggctccatcg
      721 taagcaaacc ttagaggttc tggcaaggag agagatggct ccaggaaatg ggggtgtgtc
      781 accagataag gaatctgcct aacaggaggt gggggttaga cccaatatca ggagactagg
      841 aaggaggagg cctaaggatg gggcttttct gtcaccaatc ctgtccctag taaagcttag
      901 tactgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      961 gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta tgtc
//
