LOCUS       sequence-147957-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY destination vector for site-specific integration into the 
            human AAVS1/PPP1R12C locus for doxycycline-inducible gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Hygro-iDEST
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(124..248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    410..491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(447..466)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    498..722
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     711..728
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
       61 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
      121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
      241 aaagtggttg atccggggat cctctagtca gcgtttatct gcagaattcg cccttgttta
      301 aacgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      361 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      421 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      481 tttttcactg cctcgaccca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      541 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      601 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      661 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      721 agtcgaggct gatcagcggg tttaaacggg ccctctagac tattcctttg ccctcggacg
      781 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
      841 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
      901 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
      961 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     1021 ctccggatgc ctccgctcga agtagcgc
//
