LOCUS       sequence-147958-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY destination vector for site-specific integration into the 
            human AAVS1/PPP1R12C locus for doxycycline-inducible gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Hygro-iDEST
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(104..110,111..128,129..134))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(159..283)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(300..614)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(303..327)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    374..392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    410..428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    445..463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    481..499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    517..535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    552..570
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    588..606
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
       61 ttagctcctg aaaatctcga cggatcctaa ctcaaaatcc acacattata cgagccggaa
      121 gcataaagtg taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg
      181 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      241 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgttgatcgg aattctccag
      301 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccta
      361 cctcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt ctctatcact
      421 gatagggata aactcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt
      481 tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa
      541 ctcgacatcg ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga
      601 tagggagtaa actcgacagt actaagctct agagggttaa ttaaactagg gacaggattg
      661 gtgacagaaa agccccatcc ttaggcctcc tccttcctag tctcctgata ttgggtctaa
      721 cccccacctc ctgttaggca gattccttat ctggtgacac acccccattt cctggagcca
      781 tctctctcct tgccagaacc tctaaggttt gcttacgatg gagccagaga ggatcctggg
      841 agggagagct tggcaggggg tgggagggaa gggg
//
