LOCUS       sequence-147962-         770 bp    DNA     linear   SYN 15-DEC-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(393..409)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(393..409)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(446..464)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(485..501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(485..501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(498..520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    509..525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(533..539,540..557,558..563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    578..599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(716..733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gacggtatcg ataagcttga ttttttaact attgctgagc tttattcctt catatgaaat
       61 ccaaacagac tgcctgaaag ccgacatgca gttgtgtttc ctcgtagcat ttaaacacaa
      121 taggaagaat aacacgctct cctcatctct ggggtctcac caccctaaaa aaaatccccc
      181 caatcttctt tattggcagc acgttcaagc ttaataaccc aaccgaaaac acctctatat
      241 ggtcatgaca taattagatc agaacattca aacagaagtg ggcagtgcaa agacaataca
      301 atagagggaa ataaagggca ggttcacagt ttacaggcta tgaagcaata gcagaattac
      361 gggaccagga atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac
      421 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
      481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      661 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
//
