LOCUS       sequence-147963-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  acta2:GFP transgenesis construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Whitesell TR, Kennedy RM, Carter AD, Rollins EL, Georgijevic S, 
            Santoro MM, Childs SJ
  TITLE     An alpha-smooth muscle actin (acta2/alphasma) zebrafish transgenic 
            line marking vascular mural cells and visceral smooth muscle cells.
  JOURNAL   PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590.
            eCollection 2014.
  PUBMED    24594685
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-3"; volume: "9(3):e90590. doi"; pages: " 
            10.1371/journal.pone.0090590. eCollection 2014"
COMMENT     Sequence Label: acta2_GFP_pA_pDestTolpA2
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     95..111
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    131..151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct
       61 ggtgtctgaa acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac
      121 gccaagctat caactttgta tagaaaagtt ggccacctag cttctctcac ctccttctct
      181 ttctattttg caagctttat ctgattactc ttgaaactcg ctgtgtaaag ggtcaaagaa
      241 gtgctgttgt tgttgttgtt gttttttgca caggtgtaag ctggctttaa atattttggc
      301 tggttagagt gttttctgag aaagatttga tctacatttc tgtaaatgtg attgtgagga
      361 ttgattttcc tagagacgtg ccttctgaaa gatacatttt ctacctatct tccttttctt
      421 taaagaagca cttaaagttt taaagaacaa ttctgcatgg ttgtgtctaa agcatccata
      481 ttttagtatc tttattgtga ttttatagta aaatctctgt gggtgtgagt gttttttaaa
      541 gagaggttct gaatggctgt tgaagcctgt ctgatttgtt aggtttttga ctaaatatgg
      601 ttcttctgga ccgctgacca gtcccctctt ttcctcaccc actctctctt ttttcataaa
      661 ctattaggca gagagagact gagagaggga gagaccaaag acagacatgc ttccccatca
      721 tttccaaatt aggaagttt
//
