LOCUS       sequence-147964-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  acta2:mCherry transgenesis construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Whitesell TR, Kennedy RM, Carter AD, Rollins EL, Georgijevic S, 
            Santoro MM, Childs SJ
  TITLE     An alpha-smooth muscle actin (acta2/alphasma) zebrafish transgenic 
            line marking vascular mural cells and visceral smooth muscle cells.
  JOURNAL   PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590.
            eCollection 2014.
  PUBMED    24594685
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-3"; volume: "9(3):e90590. doi"; pages: " 
            10.1371/journal.pone.0090590. eCollection 2014"
COMMENT     Sequence Label: acta2_mCherry_pA_pDestTolpA2
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     93..109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     93..109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    129..149
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtgtattagt cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg
       61 tgtctgaaac acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc
      121 caagctatca actttgtata gaaaagttgg ccacctagct tctctcacct ccttctcttt
      181 ctattttgca agctttatct gattactctt gaaactcgct gtgtaaaggg tcaaagaagt
      241 gctgttgttg ttgttgttgt tttttgcaca ggtgtaagct ggctttaaat attttggctg
      301 gttagagtgt tttctgagaa agatttgatc tacatttctg taaatgtgat tgtgaggatt
      361 gattttccta gagacgtgcc ttctgaaaga tacattttct acctatcttc cttttcttta
      421 aagaagcact taaagtttta aagaacaatt ctgcatggtt gtgtctaaag catccatatt
      481 ttagtatctt tattgtgatt ttatagtaaa atctctgtgg gtgtgagtgt tttttaaaga
      541 gaggttctga atggctgttg aagcctgtct gatttgttag gtttttgact aaatatggtt
      601 cttctggacc gctgaccagt cccctctttt cctcacccac tctctctttt ttcataaact
      661 attaggcaga gagagactga gagagggaga gaccaaagac agacatgctt ccccatcatt
      721 tccaaattag gaagttttcc catggtgctc tcagcaaggg tctt
//
