LOCUS       sequence-147970-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses-rsGreen0.9 in E. coli strains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Duwe S, De Zitter E, Gielen V, Moeyaert B, Vandenberg W, Grotjohann 
            T, Clays K, Jakobs S, Van Meervelt L, Dedecker P
  TITLE     Expression-Enhanced Fluorescent Proteins Based on Enhanced Green 
            Fluorescent Protein for Super-resolution Microscopy.
  JOURNAL   ACS Nano. 2015 Oct 27;9(10):9528-41. doi: 10.1021/acsnano.5b04129. 
            Epub 2015 Sep 9.
  PUBMED    26308583
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04129"; journalName: "ACS Nano"; date: 
            "2015-10-27- 27"; volume: "9"; issue: "10"; pages: "9528-41"
COMMENT     Sequence Label: pRSETb-rsGreen0.9
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..99
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(103..111,112..126)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(178..199)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aatattttgt ttactttaga aggagatata catatgcggg gttctcatca tcatcatcat
       61 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      121 gataaggatc cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      181 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgcgcggcga gggcgagggc
      241 gatgccacca acggcaagct gaccatgaag ttcatctgca ccaccggcaa gctgcccgtg
      301 ccctggccca ccctcgtgac caccctgacc tacggcgtgc tgtgcttcgc ccgctacccc
      361 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      421 cgcaccatca gcttcgagga cgacggctat tacaagaccc gcgccgaggt gaagttcgag
      481 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      541 atcctggggc acaagctgga gtacaacttc aacagccaca acgcctatat cacggccgac
      601 aagcagaaga acggcatcag gtctaacttc aagatccgcc tcaacgtcga ggacggcagc
      661 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      721 cccgacaacc actacctgag
//
