LOCUS       sequence-147974-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for knocking down p53 gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, 
            Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA
  TITLE     Growth-inhibitory and tumor- suppressive functions of p53 depend on 
            its repression of CD44 expression.
  JOURNAL   Cell. 2008 Jul 11. 134(1):62-73.
  PUBMED    18614011
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Jul 11. 134(1):62-73."
COMMENT     Sequence Label: shp53 pLKO.1 puro
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     43..63
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        45..63
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        91..317
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             318..498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    545..670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
       61 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
      121 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
      181 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
      241 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
      301 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      361 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      421 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      481 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      541 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      601 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      661 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      721 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      781 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      841 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      901 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      961 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctga
//
