LOCUS       sequence-147975-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for knocking down p53 gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, 
            Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA
  TITLE     Growth-inhibitory and tumor- suppressive functions of p53 depend on 
            its repression of CD44 expression.
  JOURNAL   Cell. 2008 Jul 11. 134(1):62-73.
  PUBMED    18614011
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Jul 11. 134(1):62-73."
COMMENT     Sequence Label: shp53 pLKO.1 puro
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    117..234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        283..793
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     712..730
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(815..834)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tcttgtggaa aggacgaaac accgactcca gtggtaatct acttcaagag agtagattac
       61 cactggagtc tttttgaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
      121 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      181 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      241 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
      301 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
      361 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
      421 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
      481 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
      541 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
      601 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
      661 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
      721 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
      781 cctctctccc cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg
      841 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
      901 ctaccccgcc acgcg
//
