LOCUS       sequence-147986-        1525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of hyperactive Tn5 transposase carrying E54K 
            and L372P mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1525)
  TITLE     Linnarsson lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psfTn5
FEATURES             Location/Qualifiers
     source          1..1525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             70..1497
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgattaccag tgcactgcat cgtgcggcgg attgggcgaa aagcgtgttt
      121 tctagtgctg cgctgggtga tccgcgtcgt accgcgcgtc tggtgaatgt tgcggcgcaa
      181 ctggccaaat atagcggcaa aagcattacc attagcagcg aaggcagcaa agccatgcag
      241 gaaggcgcgt atcgttttat tcgtaatccg aacgtgagcg cggaagcgat tcgtaaagcg
      301 ggtgccatgc agaccgtgaa actggcccag gaatttccgg aactgctggc aattgaagat
      361 accacctctc tgagctatcg tcatcaggtg gcggaagaac tgggcaaact gggtagcatt
      421 caggataaaa gccgtggttg gtgggtgcat agcgtgctgc tgctggaagc gaccaccttt
      481 cgtaccgtgg gcctgctgca tcaagaatgg tggatgcgtc cggatgatcc ggcggatgcg
      541 gatgaaaaag aaagcggcaa atggctggcc gctgctgcaa cttcgcgtct gagaatgggc
      601 agcatgatga gcaacgtgat tgcggtgtgc gatcgtgaag cggatattca tgcgtatctg
      661 caagataaac tggcccataa cgaacgtttt gtggtgcgta gcaaacatcc gcgtaaagat
      721 gtggaaagcg gcctgtatct gtatgatcac ctgaaaaacc agccggaact gggcggctat
      781 cagattagca ttccgcagaa aggcgtggtg gataaacgtg gcaaacgtaa aaaccgtccg
      841 gcgcgtaaag cgagcctgag cctgcgtagc ggccgtatta ccctgaaaca gggcaacatt
      901 accctgaacg cggtgctggc cgaagaaatt aatccgccga aaggcgaaac cccgctgaaa
      961 tggctgctgc tgaccagcga gccggtggaa agtctggccc aagcgctgcg tgtgattgat
     1021 atttataccc atcgttggcg cattgaagaa tttcacaaag cgtggaaaac gggtgcgggt
     1081 gcggaacgtc agcgtatgga agaaccggat aacctggaac gtatggtgag cattctgagc
     1141 tttgtggcgg tgcgtctgct gcaactgcgt gaatctttta ctccgccgca agcactgcgt
     1201 gcgcagggcc tgctgaaaga agcggaacac gttgaaagcc agagcgcgga aaccgtgctg
     1261 accccggatg aatgccaact gctgggctat ctggataaag gcaaacgcaa acgcaaagaa
     1321 aaagcgggca gcctgcaatg ggcgtatatg gcgattgcgc gtctgggcgg ctttatggat
     1381 agcaaacgta ccggcattgc gagctggggt gcgctgtggg aaggttggga agcgctgcaa
     1441 agcaaactgg atggctttct ggccgcgaaa gacctgatgg cgcagggcat taaaatctga
     1501 cagtaaaggt ggatacggat ccgaa
//
