LOCUS       sequence-148010-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Tshz2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Tshz2
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(676..694)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(677..694)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(712..728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(712..728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(725..747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    736..752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(760..766,767..784,785..790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    805..826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccggccgcca tggcggccgc gggaattcga ttcagcagaa cttgccttcc cggtctgtct
       61 ctaagcccag cctattcagc tccgtgcagc tataccggca gagcagtaag ttgtgtggct
      121 cggtgttcac cggcgccagc aggttccgct gcaggcagtg cagtgcggcc tacgacactc
      181 tcgtggagct gaccgtgcac atgaacgaga caggccacta tcaagatgac aaccgcaaaa
      241 aagataagct cagacccacg agctactcca agccccgaaa aagggccttc caagacatgg
      301 acaaagagga tgcccaaaag gtcctgaaat gtatgttttg tggtgactcc ttcgattccc
      361 tgcaagattt gagtgtccac atgataaaga caaaacatta ccaaaaagtg cctttgaagg
      421 agccggtacc aaccatttcc tccaagatgg tcacaccagc caagaagcgt gttttcgatg
      481 tcaacaggcc ttgctcccct gactccacca cagggtccct tgcagattcc ttttcttccc
      541 agaagagtgc taacctgcag ctgccctcca acagccgcta cggctaccaa aacggaatca
      601 ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat
      661 gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct
      721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      781 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgt
//
