LOCUS       sequence-148016-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression of Cas9 and gRNA empty backbone; Basta resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim 
            JS, Kim SG
  TITLE     A simple, flexible and high-throughput cloning system for plant 
            genome editing via CRISPR-Cas system.
  JOURNAL   J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
  PUBMED    26946469
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474."
COMMENT     Sequence Label: pBAtC
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             292..312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             313..339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             313..339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(344..368)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    688..712
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agcaagcgcg tgatcctggc cgacgccaac ctggacaagg tgctgagcgc ctacaacaag
       61 caccgcgaca agcccatccg cgagcaggcc gagaacatca tccacctgtt caccctgacc
      121 aacctgggcg cccccgccgc cttcaagtac ttcgacacca ccatcgaccg caagcgctac
      181 accagcacca aggaggtgct ggacgccacc ctgatccacc agagcatcac cggtctgtac
      241 gagacccgca tcgacctgag ccagctgggc ggcgacggcg gctccggacc tccaaagaaa
      301 aagagaaaag tataccccta cgacgtgccc gactacgcct aacacccagc tttcttgtac
      361 aaagtggtga tatcccgcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca
      421 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
      481 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
      541 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
      601 cctgcaggca tgcgacgtcg ggcccaagct tagcttgagc ttggatcaga ttgtcgtttc
      661 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaa
//
