LOCUS       sequence-148032-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNAs (for SpCas9) targeting 5' of Ai9 stop cassette and 
            Dmd intron 22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pCRII-Topo U6-SpAi9L-U6SpDMDL
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        70..318
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     protein_bind    complement(437..461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        574..822
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
ORIGIN
        1 tgtacaaaaa agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc
       61 gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
      121 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
      181 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      241 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      301 tggaaaggac gaaacaccga aagaattgat ttgataccgg tttaagagct atgctggaaa
      361 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      421 ggtgcttttt ttctagaccc agctttcttg tacaaagttg gcattatgag ggggagacga
      481 gaagggcgaa ttctgcagcg tccctgtaca aaaaagcagg ctttaaagga accaattcag
      541 tcgactggat ccggtaccaa ggtcgggcag gaagagggcc tatttcccat gattccttca
      601 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
      661 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      721 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      781 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggaataat ttctattata
      841 ttacagttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
      901 tcaacttgaa aaagtggcac cgagtcggtg cttttttt
//
