LOCUS       sequence-148035-        1395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 U6-SaAi9L-U6-SaAi9R
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        316..564
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        800..1048
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     repeat_region   1261..1390
                     /label=AAV2 ITR
ORIGIN
        1 cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt
       61 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      121 ggttccttgt agttaatgat taacccgcca tgctacttat ctaccggatc cactagtaac
      181 ggccgccagt gtgctggaat tcgcccttat attagaattc gcggccgcta gcccgcggct
      241 cgtccctgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
      301 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
      361 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
      421 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      481 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      541 atcttgtgga aaggacgaaa caccgctcta gagtcgcaga tcctcgttta agtactctgt
      601 gctggaaaca gcacagaatc tacttaaaca aggcaaaatg ccgtgtttat ctcgtcaact
      661 tgttggcgag atttttttta taagcttcct gtacagaatt cgtctgcaag ggcgaattct
      721 gcagcgtccc tgtacaaaaa agcaggcttt aaaggaacca attcagtcga ctggatccgg
      781 taccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg
      841 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
      901 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      961 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     1021 atatatcttg tggaaaggac gaaacaccga cgaagttata ttaagggttg tttaagtact
     1081 ctgtgctgga aacagcacag aatctactta aacaaggcaa aatgccgtgt ttatctcgtc
     1141 aacttgttgg cgagattttt tttataagct tcctgtacag aattcgtctg caagggcgaa
     1201 ttctctcgag catgcatcta gaggtagata agtagcatgg cgggttaatc attaactaca
     1261 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     1321 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     1381 gagcgcgcag cctta
//
