LOCUS       sequence-148036-        1395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 
            and 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 U6-SaDMDR7-U6-SaDMDL2
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   3..132
                     /label=AAV2 ITR
     promoter        321..569
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        805..1053
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     repeat_region   1266..1395
                     /label=AAV2 ITR
ORIGIN
        1 ggctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct
       61 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
      121 ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacc ggatccacta
      181 gtaacggccg ccagtgtgct ggaattcgcc cttatattag aattcgcggc cgctagcccg
      241 cggctcgtcc ctgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg
      301 gtaccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat ttgcatatac
      361 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
      421 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
      481 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
      541 tatatatctt gtggaaagga cgaaacaccg cagtaatgtg tcataccttc gtttaagtac
      601 tctgtgctgg aaacagcaca gaatctactt aaacaaggca aaatgccgtg tttatctcgt
      661 caacttgttg gcgagatttt ttttataagc ttcctgtaca gaattcgtct gcaagggcga
      721 attctgcagc gtccctgtac aaaaaagcag gctttaaagg aaccaattca gtcgactgga
      781 tccggtacca aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat
      841 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
      901 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      961 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     1021 gctttatata tcttgtggaa aggacgaaac accgatataa tagaaattat tcatgtttaa
     1081 gtactctgtg ctggaaacag cacagaatct acttaaacaa ggcaaaatgc cgtgtttatc
     1141 tcgtcaactt gttggcgaga ttttttttat aagcttcctg tacagaattc gtctgcaagg
     1201 gcgaattctc tcgagcatgc atctagaggt agataagtag catggcgggt taatcattaa
     1261 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     1321 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     1381 cgagcgagcg cgcag
//
