LOCUS       sequence-148042-       11133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors for expression of Citrine fluorescent protein with a 
            peroxisome localization signal in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Ogasawara Y, Ishizaki K, Kohchi T, Kodama Y
  TITLE     Cold-induced organelle relocation in the liverwort Marchantia 
            polymorpha L.
  JOURNAL   Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085.
  PUBMED    23421791
REFERENCE   2  (bases 1 to 11133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Environ. 2013 Feb 20. doi: 10.1111/pce.12085."
COMMENT     Sequence Label: pMpGWB102-Citrine-PTS1
FEATURES             Location/Qualifiers
     source          1..11133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1206..1208,1209..1211,1212..1922)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1251..1272)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1512..1531)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1859..1880
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1936..1960)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2279..2295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2279..2295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2292..2314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2303..2319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2327..2333,2334..2351,2352..2357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2372..2393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    2478..2652
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2693..3718)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(3785..4460)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    4655..4679
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5200..5991
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6237..6825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6726..6745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6979..6996
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7011..7151
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7077..7096)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7237..7259
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7495..7689
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7755..8828)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9257..9886)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1261 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1321 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1381 ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt cgcccgctac cccgaccaca
     1441 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1501 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1561 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1621 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1681 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1741 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1801 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1861 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1921 agtccaagtt gtgagaccca gctttcttgt acaaagtggt tgataacagc gcttagagct
     1981 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     2041 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     2101 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     2161 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     2221 cggtgtcatc tatgttacta gaatcgggaa atggttccgg aaccaattcg taatcatggt
     2281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa
     2401 ttaggcgcgc ctatttctga attcaacaca ttgcggacgt ttttaatgta ctgaattaac
     2461 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
     2521 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     2581 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
     2641 attatggaga aactcgagct tgtcgatcga cagatcccgg tcggcatcta ctctatttct
     2701 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
     2761 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
     2821 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
     2881 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg
     2941 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
     3001 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
     3061 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
     3121 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
     3181 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
     3241 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
     3301 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
     3361 ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca
     3421 acgtgacacc ctgtgaacgg cgggagatgc aataggtcag gctctcgcta aactccccaa
     3481 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
     3541 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
     3601 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
     3661 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
     3721 ctcattgccc cccggatctg cgaaagctcg agagagatag atttgtagag agagactggt
     3781 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga
     3841 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     3901 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     3961 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     4021 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     4081 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     4141 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     4201 caccatgttc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     4261 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     4321 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     4381 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     4441 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     4501 tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa
     4561 accgaattca gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     4621 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     4681 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     4741 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     4801 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     4861 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     4921 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     4981 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     5041 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     5101 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     5161 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     5221 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     5281 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     5341 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     5401 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     5461 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     5521 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     5581 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     5641 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     5701 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     5761 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     5821 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     5881 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     5941 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     6001 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     6061 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     6121 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     6181 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6241 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6301 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6361 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6421 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6481 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6541 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6601 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6661 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6721 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6781 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6841 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6901 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6961 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     7021 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     7081 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     7141 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7201 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7261 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     7321 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     7381 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     7441 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     7501 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     7561 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     7621 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     7681 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     7741 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     7801 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     7861 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     7921 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     7981 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     8041 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     8101 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     8161 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     8221 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     8281 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     8341 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     8401 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     8461 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     8521 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     8581 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     8641 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     8701 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     8761 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     8821 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
     8881 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     8941 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     9001 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     9061 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     9121 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     9181 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     9241 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     9301 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     9361 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     9421 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     9481 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     9541 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
     9601 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
     9661 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
     9721 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
     9781 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
     9841 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
     9901 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
     9961 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    10021 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    10081 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    10141 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    10201 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    10261 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    10321 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    10381 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    10441 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    10501 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    10561 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    10621 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    10681 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    10741 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    10801 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    10861 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    10921 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    10981 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    11041 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    11101 catgcacata caaatggacg aacggataaa cct
//
