LOCUS       sequence-148050-        6174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Kang S, Denley A, Vanhaesebroeck B, Vogt PK
  TITLE     Oncogenic transformation induced by the p110beta, -gamma, and -delta
            isoforms of class I phosphoinositide 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1289-94. Epub 2006 Jan 
            23.
  PUBMED    16432180
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2006-01-31"; volume: "103(5)"; pages: 
            "1289-94. Epub 2006 Jan 23"
COMMENT     Sequence Label: pBSFI-p110-beta
FEATURES             Location/Qualifiers
     source          1..6174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             300..308
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(1607..1618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3359..3378)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3360..3378)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3388..3405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3388..3404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3397..3419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3545..4001)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3683..3704)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3895..3914
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4028..4132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4133..4201,4202..4993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4351..4370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5164..5752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5653..5672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5906..5923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6040..6061
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6076..6081,6082..6099,6100..6106)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6114..6130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6119..6141
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6138..6154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6138..6154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(6173..6174,1..19)
                     /label=T3
                     /note="T3 promoter, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctggcc attacggccg cggccgcctc
       61 tagaggatcc gatatcgaat tcgccaccat gggatgcttc agtttcataa tgcctcctgc
      121 tatggcagac atccttgaca tctgggcggt ggattcacag atagcatctg atggctccat
      181 acctgtggat ttccttttgc ccactgggat ttatatccag ttggaggtac ctcgggaagc
      241 taccatttct tatattaagc agatgttatg gaagcaagtt cacaattacc caatgttcaa
      301 cctccttatg gatattgact cctatatgtt tgcatgtgtg aatcagactg ctgtatatga
      361 ggagcttgaa gatgaaacac gaagactctg tgatgtcaga ccttttcttc cagttctcaa
      421 attagtgaca agaagttgtg acccagggga aaaattagac tcaaaaattg gagtccttat
      481 aggaaaaggt ctgcatgaat ttgattcctt gaaggatcct gaagtaaatg aatttcgaag
      541 aaaaatgcgc aaattcagcg aggaaaaaat cctgtcactt gtgggattgt cttggatgga
      601 ctggctaaaa caaacatatc caccagagca tgaaccatcc atccctgaaa acttagaaga
      661 taaactttat gggggaaagc tcatcgtagc tgttcatttt gaaaactgcc aggacgtgtt
      721 tagctttcaa gtgtctccta atatgaatcc tatcaaagta aatgaattgg caatccaaaa
      781 acgtttgact attcatggga aggaagatga agttagcccc tatgattatg tgttgcaagt
      841 cagcgggaga gtagaatatg tttttggtga tcatccacta attcagttcc agtatatccg
      901 gaactgtgtg atgaacagag ccctgcccca ttttatactt gtggaatgct gcaagatcaa
      961 gaaaatgtat gaacaagaaa tgattgccat agaggctgcc ataaatcgaa attcatctaa
     1021 tcttcctctt ccattaccac caaagaaaac acgaattatt tctcatgttt gggaaaataa
     1081 caaccctttc caaattgtct tggttaaggg aaataaactt aacacagagg aaactgtaaa
     1141 agttcatgtc agggctggtc tttttcatgg tactgagctc ctgtgtaaaa ccatcgtaag
     1201 ctcagaggta tcagggaaaa atgatcatat ttggaatgaa ccactggaat ttgatattaa
     1261 tatttgtgac ttaccaagaa tggctcgatt atgttttgct gtttatgcag ttttggataa
     1321 agtaaaaacg aagaaatcaa cgaaaactat taatccctct aaatatcaga ccatcaggaa
     1381 agctggaaaa gtgcattatc ctgtagcgtg ggtaaatacg atggtttttg actttaaagg
     1441 acaattgaga actggagaca taatattaca cagctggtct tcatttcctg atgaactcga
     1501 agaaatgttg aatccaatgg gaactgttca aacaaatcca tatactgaaa atgcaacagc
     1561 tttgcatgtt aaatttccag agaataaaaa acaaccttat tattaccctc ccttcgataa
     1621 gattattgaa aaggcagctg agattgcaag cagtgatagt gctaatgtgt caagtcgagg
     1681 tggaaaaaag tttcttcctg tattgaaaga aatcttggac agggatccct tgtctcaact
     1741 gtgtgaaaat gaaatggatc ttatttggac tttgcgacaa gactgccgag agattttccc
     1801 acaatcactg ccaaaattac tgctgtcaat caagtggaat aaacttgagg atgttgctca
     1861 gcttcaggcg ctgcttcaga tttggcctaa actgcccccc cgggaggccc tagagcttct
     1921 ggatttcaac tatccagacc agtacgttcg agaatatgct gtaggctgcc tgcgacagat
     1981 gagtgatgaa gaactttctc aatatctttt acaactggtg caagtgttaa aatatgagcc
     2041 ttttcttgat tgtgccctct ctagattcct attagaaaga gcacttggta atcggaggat
     2101 agggcagttt ctattttggc atcttaggtc agaagtgcac attcctgctg tctcagtaca
     2161 atttggtgtc atccttgaag catactgccg gggaagtgtg gggcacatga aagtgctttc
     2221 taagcaggtt gaagcactca ataagttaaa aactttaaat agtttaatca aactgaatgc
     2281 cgtgaagtta aacagagcca aagggaagga ggccatgcat acctgtttaa aacagagtgc
     2341 ttaccgggaa gccctctctg acctgcagtc acccctgaac ccatgtgtta tcctctcaga
     2401 actctatgtt gaaaagtgca aatacatgga ttccaaaatg aagcctttgt ggctggtata
     2461 caataacaag gtatttggtg aggattcagt tggagtgatt tttaaaaatg gtgatgattt
     2521 acgacaggat atgttgacac tccaaatgtt gcgcttgatg gatttactct ggaaagaagc
     2581 tggtttggat cttcggatgt tgccttatgg ctgtttagca acaggagatc gctctggcct
     2641 cattgaagtt gtgagcacct ctgaaacaat tgctgacatt cagctgaaca gtagcaatgt
     2701 ggctgctgca gcagccttca acaaagatgc ccttctgaac tggcttaaag aatacaactc
     2761 tggggatgac ctggaccgag ccattgagga atttacactg tcctgtgctg gctactgtgt
     2821 agcttcttat gtccttggga ttggtgacag acatagtgac aacatcatgg tcaaaaaaac
     2881 tggccagctc ttccacattg actttggaca tattcttgga aatttcaaat ctaagtttgg
     2941 cattaaaagg gagcgagtgc cttttattct tacctatgat ttcatccatg tcattcaaca
     3001 aggaaaaaca ggaaatacag aaaagtttgg ccggttccgc cagtgttgtg aggatgcata
     3061 tctgatttta cgacggcatg ggaatctctt catcactctc tttgcgctga tgttgactgc
     3121 agggcttcct gaactcacat cagtcaaaga tatacagtat cttaaggact ctcttgcatt
     3181 agggaagagt gaagaagaag cactcaaaca gtttaagcaa aaatttgatg aggcgctcag
     3241 ggaaagctgg actactaaag tgaactggat ggcccacaca gttcggaaag actacagatc
     3301 ttaacgatca agcttatcga tgtcgacggg cccggccgcc tcggccgtac ccaattcgcc
     3361 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3421 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3481 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3541 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3601 tcattttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3661 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3721 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3781 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3841 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3901 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3961 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     4021 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4081 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4141 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4201 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4261 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4321 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4381 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4441 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4501 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4561 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4621 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4681 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4741 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4801 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4861 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4921 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4981 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5041 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5101 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5161 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5221 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5281 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5341 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5401 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5461 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5521 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5581 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5641 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5701 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5761 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5821 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5881 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5941 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6001 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6061 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6121 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgc
//
