LOCUS       sequence-148060-       11365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Sen GL, Reuter JA, Webster DE, Zhu L, Khavari PA
  TITLE     DNMT1 maintains progenitor function in self-renewing somatic tissue.
  JOURNAL   Nature. 2010 Jan 28. 463(7280):563-7.
  PUBMED    20081831
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 28. 463(7280):563-7."
COMMENT     Sequence Label: LZRS-GADD45B
FEATURES             Location/Qualifiers
     source          1..11365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1303..1677
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(1349..1365)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(1687..2103)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1703..1719)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1735..1757)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2168..2525
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             2590..3059
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        3596..3700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3701..3769,3770..4561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3919..3938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5055..6844
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        8876..9375
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8911..8934)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9308..9327
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9458..10057
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9458..9477)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9954..9974
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10287..10421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10324..10343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10378..10397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10544..11132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11033..11052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11286..11303
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctca
      781 gcgttcctga agagagatgt aggggaccca ctggttgttg ccccggcttt cttcgcagta
      841 gctggccacc tccaccaagc cgtggctctt ccaggcgtcc gtgtgagggt tcgtgaccag
      901 gagacaatgc aggtctcggg cctcggtggt gccctgggtc tcggccggct ctcccaggag
      961 ctgcgccagg cgctgcatgc ccgacacccg cacgatgttg atgtcgttgt cacagcagaa
     1021 ggactggatg agcgtgaagt ggatttgcag ggcgatgtca tcctcctcct cctcgtcaat
     1081 ggccaagagg cagaggacca cgctgtctgg gtccacattc atcaacttgg ccgactcgta
     1141 cacccccact gtgaggcgat cctggcgctg agcggccacc aaaagctcct ccaccgcggc
     1201 ggtcaccgtc tgcatcttct gcgccgcgtt gtcgcacgcc acgagctctt ccagcgtcat
     1261 ggtggcaagc ttgaattccc gtaccaccac actgggatcc ggcagtctag aggatggtcc
     1321 acccccgggg tcggcagcct tcacgtgggc ggcgtgtatc caagctgcga tgccgtctac
     1381 tttgagggcg gtgggggtgg tcagcaggac tgtgtaaggt cctttccagc gaggttctag
     1441 gttcttagtc tggtgtcggc ggacccacac tgtgtcgccg actcggtaag ggtgaggtac
     1501 caccggtcgg tccagttgtt cttggtaggc tgccgccaga ggtctccaga cttcgtgctg
     1561 gactaagtag agagcctgta agtgagcttg gagagagggg ctgttagtaa ctcttgtcaa
     1621 gtcagggtca gggaagttta caaggggcgg gggtgcccca tataagatct catatggcca
     1681 tatgggggcg cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga
     1741 acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg
     1801 gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat
     1861 gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg
     1921 ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca
     1981 ttctgcagag cagaaggtaa cccaacgtct cttcttgaca actaccgact ggttgtgagc
     2041 gatccgctcg acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg
     2101 gcccgggccc aaattttcag acaaatacag aaacacagtc agacagagac aacacagaac
     2161 gatgctgcag cagacaagac gcgcggcgcg gcttcggtcc caaaccgaaa gcaaaaattc
     2221 agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat atccctcctc
     2281 taaggggggt gcaccaaaga gtccaaaacg atcgggattt ttggactcag gtcgggccac
     2341 aaaaacggcc cccgaagtcc ctgggacgtc tcccagggtt gcggccgggt gttccgaact
     2401 cgtcagttcc accacgggtc cgccagatac agagctagtt agctaactag taccgacgca
     2461 ggcgcataaa atcagtcata gacactagac aatcggacag acacagataa gttgctggcc
     2521 agcttacctc ccggtggtgg gtcggtggtc cctgggcagg ggtctcccga tcccggacga
     2581 gcccccaaat gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa
     2641 ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac
     2701 ccgggcgact cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta
     2761 ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc
     2821 aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc
     2881 tagaaactgc tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg
     2941 gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct
     3001 tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc
     3061 tgttccatct gttcctgacc ttgatctgaa cttttctatt ctcagttatg tatttttcca
     3121 tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcac
     3181 atgtatatgt caaaaataaa aatcaactaa ttgactagta attaatatga ctggcataat
     3241 gggaaattga tcctgacaga tgcaaactgg cttctcagca gcgcatttat gttgtcaact
     3301 gaggaaggaa acgttaatga cagaaactct aagtaatttc cacgtttatc tatttttatt
     3361 tatactagct ttggtaacag gaatattgca gcattcatgc acattgaaac ccttatgaaa
     3421 taaaaacatc tgtgcattta aaatggaatt aacattttaa atgttaaaaa aagctggctt
     3481 agcttccccc cgccccctag ggcatagaac aagtcaaatg ctttatatat ttgagtttgg
     3541 gatgtattag gaaactccta agagcaaagc tggtggcact tttcggggaa atgtgcgcgg
     3601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3961 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4021 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4081 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4141 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4201 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4261 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4321 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4381 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4441 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4501 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4561 actgtcagac caagtttact catatatact ttagattgat ttgcggccag atccccgccc
     4621 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     4681 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     4741 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     4801 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     4861 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     4921 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     4981 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     5041 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     5101 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     5161 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     5221 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     5281 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     5341 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     5401 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     5461 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     5521 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     5581 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     5641 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     5701 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     5761 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     5821 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     5881 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     5941 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     6001 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     6061 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     6121 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     6181 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     6241 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     6301 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     6361 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     6421 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     6481 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     6541 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     6601 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     6661 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     6721 atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc
     6781 tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt
     6841 cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg
     6901 tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc
     6961 ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa
     7021 tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct actggggatt
     7081 tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag
     7141 ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct
     7201 ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct
     7261 actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc
     7321 ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag
     7381 accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg
     7441 gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca
     7501 gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg
     7561 acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa
     7621 ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac
     7681 caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct
     7741 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
     7801 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
     7861 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
     7921 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
     7981 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
     8041 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
     8101 gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct
     8161 cccgctcctg ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga
     8221 cgtttttggg gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg
     8281 gctcctggtc ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca
     8341 ccccctcttc tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc
     8401 tctcctaggc catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa
     8461 agagatcaat agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct
     8521 gccccctcca acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag
     8581 gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc
     8641 ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa
     8701 ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc
     8761 ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc
     8821 ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattaatt ctaccgggta
     8881 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     8941 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     9001 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     9061 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     9121 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     9181 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     9241 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     9301 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     9361 tctccgggcc tttcgacctg caggtcggcc gccacgaccg gtgccgccac catcccctga
     9421 cccacgcccc tgacccctca caaggagacg accttccatg accgagtaca agcccacggt
     9481 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     9541 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     9601 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     9661 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     9721 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     9781 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     9841 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     9901 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9961 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
    10021 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
    10081 cccgaccgaa aggagcgcac gaccccatgg ctccgaccga agccacccgg ggcggccccg
    10141 ccgaccccgc acccgccccc gaggcccacc gactctagag gatcataatc agccatacca
    10201 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    10261 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    10321 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10381 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctaattcggc cgcaaaagga
    10441 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    10501 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10561 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10621 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10681 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10741 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10801 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10861 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10921 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10981 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    11041 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    11101 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    11161 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    11221 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    11281 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    11341 ccgcgcgttg gccgattcat taatg
//
