LOCUS       sequence-148069-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination piggyBac vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   dos Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, 
            Hendrich B, Silva JC
  TITLE     MBD3/NuRD facilitates induction of pluripotency in a 
            context-dependent manner.
  JOURNAL   Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 
            10.1016/j.stem.2014.04.019. Epub 2014 May 15.
  PUBMED    24835571
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2014.04.019"; journalName: "Cell Stem Cell"; date: 
            "2014-07-3- 3"; volume: "15"; issue: "1"; pages: "102-10"
COMMENT     Sequence Label: pPB-CAG-Dest-pA-pgk-bsd
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   9..43
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    318..351
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        361..740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 taccttaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc
       61 tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct
      121 tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac
      181 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
      241 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
      301 ttcttgttat agatatcata acttcgtata atgtatgcta tacgaagtta tgcggccctc
      361 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      421 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      481 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      541 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      601 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      661 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      721 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccat
//
