LOCUS       sequence-148071-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Van Engelenburg SB, Palmer AE
  TITLE     Imaging type-III secretion reveals dynamics and spatial segregation 
            of Salmonella effectors.
  JOURNAL   Nat Methods. 2010 Apr;7(4):325-30. doi: 10.1038/nmeth.1437. Epub 
            2010 Mar 14.
  PUBMED    20228815
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1437"; journalName: "Nat Methods"; date: "2010-04"; 
            volume: "7"; issue: "4"; pages: "325-30"
COMMENT     Sequence Label: pLAMP1-mCherry
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..781)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(349..368)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     618..636
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agcggccgcc actgtgctgg atatctgcag aattccacca cactggacta gtggatccga
       61 gctcggtacc ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
      121 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
      181 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
      241 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
      301 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
      361 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
      421 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
      481 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
      541 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
      601 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
      661 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
      721 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
      781 taccggtaaa cgctagatca accact
//
