LOCUS       sequence-148073-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mKlf2 and mNanog-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   dos Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, 
            Hendrich B, Silva JC
  TITLE     MBD3/NuRD facilitates induction of pluripotency in a 
            context-dependent manner.
  JOURNAL   Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 
            10.1016/j.stem.2014.04.019. Epub 2014 May 15.
  PUBMED    24835571
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2014.04.019"; journalName: "Cell Stem Cell"; date: 
            "2014-07-3- 3"; volume: "15"; issue: "1"; pages: "102-10"
COMMENT     Sequence Label: pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctctgggca cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgaat
       61 caacaagttt gtacaaaaaa gcaggctttc gctagcgcca ccatggcgct cagcgagcct
      121 atcttgccgt cctttgccac tttcgccagc ccgtgcgagc gcggcctcca ggagcgctgg
      181 ccgcgaaatg aacccgaggc gggcggcacg gatgaggacc taaacaacgt gttggacttc
      241 atcctctcca tgggattgga cggtctgggc gccgagaatc ctcccgagcc cccgccgcag
      301 cccccgccgc ctgccttcta ctacccggag ccgggtgctc cgccgcccta cagcatcccc
      361 gcggccagcc tgggaacaga gctgctgcgc cccgacctgg acccgcctca ggggccggct
      421 ctgcacggcc gcttcctcct tgcgcctccc gggcggctag tgaaggccga gccccccgag
      481 gtggacggcg gcggctacgg ctgcgctccg ggcctggccc acggaccgcg cgggctgaag
      541 ctcgagggcg ccccaggagc gacaggtgca tgcatgcggg gtcccgccgg ccgccccccg
      601 ccgcccccgg acacgccgcc gctcagcccc gacggccccc tgcgcatccc ggcgtccggt
      661 ccacgcaacc cgttcccgcc gcccttcggt cccggcccca gcttcggcgg ccccggcccc
      721 gcgttgcact a
//
