LOCUS       sequence-148074-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mKlf2 and mNanog-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   dos Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, 
            Hendrich B, Silva JC
  TITLE     MBD3/NuRD facilitates induction of pluripotency in a 
            context-dependent manner.
  JOURNAL   Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 
            10.1016/j.stem.2014.04.019. Epub 2014 May 15.
  PUBMED    24835571
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2014.04.019"; journalName: "Cell Stem Cell"; date: 
            "2014-07-3- 3"; volume: "15"; issue: "1"; pages: "102-10"
COMMENT     Sequence Label: pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(128..181)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
       61 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatct
      121 taattaatgg gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct
      181 cgccagaacc catgtgtcgc ttcatgtgca aggccaggtg gtcggacctg gagaaggccc
      241 ggtcgcacaa gtggcactga aagggtctgt gacctgtgtg ctttcggtag tggcgggtaa
      301 gctcgtcaga gcgcgcgaac ttccagccgc atccttccca gttgcaatga taaggcttct
      361 cacctgtgtg tgtacgcaga tgcgccttta ggtgcgagct cttggtgtag gtcttgccgc
      421 agttggtgta gctgcaagta tgtgtggcgg cgcgcttgcg gggccaggag cggcggccgc
      481 gtttgggctt ggcctccagc agctccagcg gggacgaggg cggcgtgagg agaccgcgcg
      541 tggcaggtgg agccaagccc agcgccgccg ccgctgccgc cgcgtcctcg aaaagaccga
      601 aggcgccagg cgcgggaggc ccgtagtgca acgcggggcc ggggccgccg aagctggggc
      661 cgggaccgaa gggcggcggg aacgggttgc gtggaccgga cgccgggatg cgcagggggc
      721 cgtcggggct g
//
