LOCUS       sequence-148089-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV/TO SV40 small + Large T (w612-1)
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctttaaagg aaccaattca
      121 gtcgactgga tccgcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      181 cctaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
      241 tgcagctaat ggaccttcta ggtcttgaaa ggagtgcctg ggggaatatt cctctgatga
      301 gaaaggcata tttaaaaaaa tgcaaggagt ttcatcctga taaaggagga gatgaagaaa
      361 aaatgaagaa aatgaatact ctgtacaaga aaatggaaga tggagtaaaa tatgctcatc
      421 aacctgactt tggaggcttc tgggatgcaa ctgaggtatt tgcttcttcc ttaaatcctg
      481 gtgttgatgc aatgtactgc aaacaatggc ctgagtgtgc aaagaaaatg tctgctaact
      541 gcatatgctt gctgtgctta ctgaggatga agcatgaaaa tagaaaatta tacaggaaag
      601 atccacttgt gtgggttgat tgctactgct tc
//
