LOCUS       sequence-148106-         574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive dCas9-KRAB 2nd generation lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
  TITLE     Cas9 effector-mediated regulation of transcription and 
            differentiation in human pluripotent stem cells.
  JOURNAL   Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
  PUBMED    24346702
REFERENCE   2  (bases 1 to 574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-01"; volume: "141"; issue: 
            "1"; pages: "219-23"
COMMENT     Sequence Label: pHAGE EF1-alpha dCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..67)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(185..379)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(416..505)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(506..526)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccctacccgg tagaatttct agtttaatta atcattacta agcgtagtct gggacgtcgt
       61 atgggtattc gaaccgcggg ccctctagac tcgagcggcc gccactgtgc tggatatcaa
      121 ccactttgta caagaaagct gggtctagat atctcgaggc ggccgcttat ccggattcga
      181 attataccag ccaaggttct tccccctttt cgagcctaag gatcacatcc ggtttcgtca
      241 actggtatcc gagactgacg aggttcttgt aattctcaag catgacattt cgatacacaa
      301 tttgttgcgc agtatccagc aatttccact cctcacgtgt aaagtccaca aacacgtcct
      361 tgaacgtaac cagtgttctc gaccatgccg taagtgattt cgcgtccatg gatccagcgt
      421 aatctggaac gtcataagga tacgatcctg catagtccgg gacgtcatag ggatagcccg
      481 catagtcagg aacatcgtat gggtatacct tcctcttctt cttgggcccg ccggtgccgt
      541 ctccaccgag ctgagagagg tcgattcttg tttc
//
