LOCUS       sequence-148107-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive dCas9-KRAB 2nd generation lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
  TITLE     Cas9 effector-mediated regulation of transcription and 
            differentiation in human pluripotent stem cells.
  JOURNAL   Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
  PUBMED    24346702
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-01"; volume: "141"; issue: 
            "1"; pages: "219-23"
COMMENT     Sequence Label: pHAGE EF1-alpha dCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagttttttt cttccatttc aggtgtcgtg aactagtcca gtgtggtgga attctgcaga
       61 tatcaacaag tttgtacaaa aaagcaggct ggcgccacca tggacaagaa gtactccatt
      121 gggctcgcta tcggtaccaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg
      181 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
      241 attggagccc tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca
      301 gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt
      361 aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg
      421 gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg
      481 taccatgaaa agtacccaac catatatcat ctgaggaaga agctggtaga cagtactgat
      541 aaggctgact tgcggttgat ctatctcgcg ctggcgcaca tgatcaaatt tcggggacac
      601 ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa
      661 ctggttcaga cttacaatca gcttttcgag gagaacccga tcaacgcatc cggcgttgac
      721 gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca
      781 cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct gtcactcggg
      841 ctgaccccca actttaaatc taacttcgac ctggcc
//
